BIOMAT Database Logo BIOMAT Database Logo

Human chorion cells respond to growth factors but lose steroidogenic capacity in primary monolayer cell culture. 1986

K A Ferguson, and B F Mitchell, and A K Tanswell

This study has defined a method for preparation and monolayer culture of cells from chorion laeve. Cell number and cell protein content are stable over 7 d in culture. The cells will divide in response to epidermal growth factor in the presence of a supplemented, enriched medium and a collagen matrix, but they lose steroidogenic activity over time in culture. This culture system can be used as the starting point for the development of a chemically defined hormone-supplemented, serum-free culture system for studies of chorion cell differentiation and fetal membrane cell interactions.

UI MeSH Term Description Entries
D011374 Progesterone The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS. Pregnenedione,Progesterone, (13 alpha,17 alpha)-(+-)-Isomer,Progesterone, (17 alpha)-Isomer,Progesterone, (9 beta,10 alpha)-Isomer
D002454 Cell Differentiation Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs. Differentiation, Cell,Cell Differentiations,Differentiations, Cell
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D002823 Chorion The outermost extra-embryonic membrane surrounding the developing embryo. In REPTILES and BIRDS, it adheres to the shell and allows exchange of gases between the egg and its environment. In MAMMALS, the chorion evolves into the fetal contribution of the PLACENTA. Chorions
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004815 Epidermal Growth Factor A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form. EGF,Epidermal Growth Factor-Urogastrone,Urogastrone,Human Urinary Gastric Inhibitor,beta-Urogastrone,Growth Factor, Epidermal,Growth Factor-Urogastrone, Epidermal,beta Urogastrone
D004970 Estrone An aromatized C18 steroid with a 3-hydroxyl group and a 17-ketone, a major mammalian estrogen. It is converted from ANDROSTENEDIONE directly, or from TESTOSTERONE via ESTRADIOL. In humans, it is produced primarily by the cyclic ovaries, PLACENTA, and the ADIPOSE TISSUE of men and postmenopausal women. Folliculin (Hormone),Estrone, (+-)-Isomer,Estrone, (8 alpha)-Isomer,Estrone, (9 beta)-Isomer,Estrovarin,Kestrone,Unigen,Wehgen
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

K A Ferguson, and B F Mitchell, and A K Tanswell
Human mesenchymal stem cells respond to fibroblast growth factors.
March 1997, Human cell,
K A Ferguson, and B F Mitchell, and A K Tanswell
Human prepubertal testicular cells in culture: steroidogenic capacity, paracrine and hormone control.
June 1995, The Journal of steroid biochemistry and molecular biology,
K A Ferguson, and B F Mitchell, and A K Tanswell
Primary monolayer cell culture of bovine parathyroids: effects of calcium, isoproterenol and growth factors.
June 1983, Molecular and cellular endocrinology,
K A Ferguson, and B F Mitchell, and A K Tanswell
Human breast cancers respond to growth factors in vivo but not in vitro.
September 1994, Cancer letters,
K A Ferguson, and B F Mitchell, and A K Tanswell
The steroidogenic response of adult rat adrenocortical cells in monolayer culture.
March 1973, The Journal of endocrinology,
K A Ferguson, and B F Mitchell, and A K Tanswell
Growth-inhibitory effect of TGF-B on human fetal adrenal cells in primary monolayer culture.
August 1989, Journal of cellular physiology,
K A Ferguson, and B F Mitchell, and A K Tanswell
Basal serum-free culture system which supports growth of fetal rat adrenal cells in primary monolayer cell culture.
October 1988, Journal of developmental physiology,
K A Ferguson, and B F Mitchell, and A K Tanswell
Growth of sebaceous cells in monolayer culture.
February 1992, In vitro cellular & developmental biology : journal of the Tissue Culture Association,
K A Ferguson, and B F Mitchell, and A K Tanswell
Catechols in primary and metastatic human malignant melanoma cells in monolayer culture.
June 1976, European journal of cancer,
K A Ferguson, and B F Mitchell, and A K Tanswell
JAK2 contributes to the intrinsic capacity of primary hematopoietic cells to respond to stem cell factor.
February 2004, Experimental hematology,
Copied contents to your clipboard!