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To clarify the mechanism of increased numbers of megakaryocytes in patients with essential thrombocythaemia (ET), we studied in vitro megakaryocytopoiesis in ET and other myeloproliferative disorders, using a megakaryocytic colony assay in methylcellulose containing plasma or serum and medium conditioned by phytohaemagglutinin (PHA) stimulated leucocytes (PHA-LCM). Megakaryocytic colony formation was supported well by heparinized or citrated plasma and citrated serum which was harvested after clot formation of citrated plasma. Whole serum was inhibitory for megakaryocytic colony growth. The addition of platelet releasates and partially purified platelet derived growth factor (PDGF) resulted in a decrease in the number of megakaryocytic colonies. These findings suggested that platelet-derived factor(s) in serum was inhibitory to megakaryocytic colony formation. ET plasma supported the megakaryocytic colony formation by normal or ET bone marrow cells better than normal plasma. Moreover, in ET bone marrow cells, spontaneous megakaryocytic colonies were formed in the absence of PHA-LCM. Increased megakaryocytopoiesis in ET may be ascribed to (i) increased megakaryocyte-colony stimulating activity (Meg-CSA) in plasma and (ii) increased sensitivity to Meg-CSA or autonomous proliferation of megakaryocytic progenitor cells.
N Komatsu, and
T Suda, and
Y Sakata, and
M Eguchi, and
K Kaji, and
M Saito, and
Y Miura
June 1987,
British journal of haematology,
N Komatsu, and
T Suda, and
Y Sakata, and
M Eguchi, and
K Kaji, and
M Saito, and
Y Miura
January 2009,
Leukemia research,
N Komatsu, and
T Suda, and
Y Sakata, and
M Eguchi, and
K Kaji, and
M Saito, and
Y Miura
April 2004,
European journal of haematology,
N Komatsu, and
T Suda, and
Y Sakata, and
M Eguchi, and
K Kaji, and
M Saito, and
Y Miura
February 1995,
Leukemia,
N Komatsu, and
T Suda, and
Y Sakata, and
M Eguchi, and
K Kaji, and
M Saito, and
Y Miura
February 1989,
British journal of haematology,
N Komatsu, and
T Suda, and
Y Sakata, and
M Eguchi, and
K Kaji, and
M Saito, and
Y Miura
July 1985,
Journal of cellular physiology,
N Komatsu, and
T Suda, and
Y Sakata, and
M Eguchi, and
K Kaji, and
M Saito, and
Y Miura
November 1986,
The Journal of laboratory and clinical medicine,
N Komatsu, and
T Suda, and
Y Sakata, and
M Eguchi, and
K Kaji, and
M Saito, and
Y Miura
July 1988,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
N Komatsu, and
T Suda, and
Y Sakata, and
M Eguchi, and
K Kaji, and
M Saito, and
Y Miura
April 1996,
Zhonghua yi xue za zhi,
N Komatsu, and
T Suda, and
Y Sakata, and
M Eguchi, and
K Kaji, and
M Saito, and
Y Miura
February 2000,
Leukemia & lymphoma,
