-transparent.png){kind=logo}
The effects of the monoclonal antibody-mitomycin C conjugate against human colon cancers were studied, in vitro and in vivo. Mitomycin C (MMC) was conjugated with the human colon cancer-specific monoclonal antibody, using a cyanogen bromide method. The effect of the conjugate and free MMC, in vitro, was measured by incubation with human colon cancer SW1116 cells. The MMC concentration of the conjugate and of free MMC needed for 50 per cent killing of target cells was 0.11 microgram/ml and 7.00 micrograms/ml, respectively. The effect of the conjugate and of free MMC, in vivo, was assayed by the growth inhibition of human colon cancer xenografted in nude mice, after intraperitoneal injections of 30 micrograms MMC/kg. Antitumor effects of the conjugate against the cancer in nude mice were significantly more potent than that of free MMC.
H Kotanagi, and
K Fukuda, and
N Ogata, and
M Takahashi, and
T Masuda, and
K Koyama, and
T Takahashi
April 1986,
The Tohoku journal of experimental medicine,
H Kotanagi, and
K Fukuda, and
N Ogata, and
M Takahashi, and
T Masuda, and
K Koyama, and
T Takahashi
February 1991,
Japanese journal of cancer research : Gann,
H Kotanagi, and
K Fukuda, and
N Ogata, and
M Takahashi, and
T Masuda, and
K Koyama, and
T Takahashi
July 1990,
Zhongguo yao li xue bao = Acta pharmacologica Sinica,
H Kotanagi, and
K Fukuda, and
N Ogata, and
M Takahashi, and
T Masuda, and
K Koyama, and
T Takahashi
January 1990,
Bioconjugate chemistry,
H Kotanagi, and
K Fukuda, and
N Ogata, and
M Takahashi, and
T Masuda, and
K Koyama, and
T Takahashi
February 1994,
Cancer,
H Kotanagi, and
K Fukuda, and
N Ogata, and
M Takahashi, and
T Masuda, and
K Koyama, and
T Takahashi
January 1985,
Biochemical pharmacology,
H Kotanagi, and
K Fukuda, and
N Ogata, and
M Takahashi, and
T Masuda, and
K Koyama, and
T Takahashi
January 1989,
Yao xue xue bao = Acta pharmaceutica Sinica,
H Kotanagi, and
K Fukuda, and
N Ogata, and
M Takahashi, and
T Masuda, and
K Koyama, and
T Takahashi
March 1996,
[Hokkaido igaku zasshi] The Hokkaido journal of medical science,
H Kotanagi, and
K Fukuda, and
N Ogata, and
M Takahashi, and
T Masuda, and
K Koyama, and
T Takahashi
November 1985,
Cancer research,
H Kotanagi, and
K Fukuda, and
N Ogata, and
M Takahashi, and
T Masuda, and
K Koyama, and
T Takahashi
June 1980,
Cancer research,
