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Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques. 2021
Dina M Metwally, and
Reem Alajmi, and
Muslimah N Alsulami, and
Isra M Al-Turaiki, and
Rewaida Abdel-Gaber, and
Afrah F Alkhuriji, and
Haleema H Albohiri, and
Khalil Mohamed, and
Hanadi B Baghdadi, and
Manal F El-Khadragy, and
Guillermo T Isaias, and
Saeed El-Ashram
Department of Parasitology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
BACKGROUND Thileriosis is a tick -born disease caused by hemoprotozoan parasites which has global veterinary and economic implications. METHODS Blood samples were collected from 216 sheep and 83 goats from Jeddah, Saudi Arabia, were analyzed to determine whether the animals were infected with Theileria spp. parasites. The parasites were detected using a polymerase chain reaction (PCR) targeting the gene of 18S rRNA followed by sequencing. RESULTS According to obtained findings, Theileria spp. were detected in sheep (57.8%, 48/83) and goats (51.9%, 112/216). Phylogenetic analysis to sequence data showed that T. ovis identified in this study were found to be closely connected to an isolate from Turkey, with 84.4-99.8% pairwise identity and 52.35-99.79% coverage.
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