Recent advances in fluorescent probes for lipid droplets. 2022

Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. shiwen@iccas.ac.cn.

Lipid droplets (LDs) have been known as a non-negligible cellular organelle for lipid storage and metabolism. Fluorescent probes for imaging LDs would be paramount for depicting their functions in cells. Although commercially available Nile Red and BODIPYtm 493/503 have been widely used for labelling LDs, they exhibit unsatisfactory specificity and spectroscopic properties. This feature article reviews the recent advances in organic fluorescent probes for imaging LDs. We first introduce the key points for probe design, including regulating hydrophobicity and enhancing fluorescence quantum yield in LDs. Then, we summarize the structural features and biological applications of some representative LD probes classified by their frameworks. In addition, the current challenges and future research trends for the fluorescent probes of LDs are discussed as well.

UI MeSH Term Description Entries
D008856 Microscopy, Fluorescence Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye. Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D011084 Polycyclic Aromatic Hydrocarbons Aromatic hydrocarbons that contain extended fused-ring structures. Polycyclic Aromatic Hydrocarbon,Polycyclic Hydrocarbons, Aromatic,Polynuclear Aromatic Hydrocarbon,Polynuclear Aromatic Hydrocarbons,Aromatic Hydrocarbon, Polycyclic,Aromatic Hydrocarbon, Polynuclear,Aromatic Hydrocarbons, Polycyclic,Aromatic Hydrocarbons, Polynuclear,Aromatic Polycyclic Hydrocarbons,Hydrocarbon, Polycyclic Aromatic,Hydrocarbon, Polynuclear Aromatic,Hydrocarbons, Aromatic Polycyclic,Hydrocarbons, Polycyclic Aromatic,Hydrocarbons, Polynuclear Aromatic
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003374 Coumarins Synthetic or naturally occurring substances related to coumarin, the delta-lactone of coumarinic acid. 1,2-Benzopyrone Derivatives,1,2-Benzopyrones,Coumarin Derivative,Coumarine,1,2-Benzo-Pyrones,Benzopyran-2-ones,Coumarin Derivatives,Coumarines,1,2 Benzo Pyrones,1,2 Benzopyrone Derivatives,1,2 Benzopyrones,Benzopyran 2 ones,Derivative, Coumarin,Derivatives, 1,2-Benzopyrone,Derivatives, Coumarin
D005456 Fluorescent Dyes Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags. Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D047309 Flavones A group of 4-keto-FLAVONOIDS. 2-Phenyl-2-Ene-Benzopyran-4-One Compounds
D053644 Naphthalimides Compounds with three fused rings that appear like a naphthalene fused to piperidone or like a benz(de)isoquinoline-1,3-dione (not to be confused with BENZYLISOQUINOLINES which have a methyl separating the naphthyl from the benzyl rings). Members are CYTOTOXINS. Naphthalimide
D066292 Lipid Droplets Dynamic cytoplasmic organelles found in almost all cells. They consist of a central core of LIPIDS surrounded by a phospholipid monolayer studded with surface proteins, and are involved in LIPID METABOLISM and storage. Adiposomes,Lipid Bodies,Lipid Storage Bodies,Oil Bodies,Oleosomes,Spherosomes,Adiposome,Droplet, Lipid,Droplets, Lipid,Lipid Body,Lipid Droplet,Lipid Storage Body,Oil Body,Oleosome,Spherosome,Storage Bodies, Lipid,Storage Body, Lipid

Related Publications

Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
September 2018, Materials (Basel, Switzerland),
Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
October 2022, Biosensors,
Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
October 2023, Organic & biomolecular chemistry,
Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
January 2021, Molecules (Basel, Switzerland),
Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
January 2017, Molecules (Basel, Switzerland),
Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
March 2024, Molecules (Basel, Switzerland),
Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
April 2024, Current opinion in chemical biology,
Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
January 2021, Frontiers in chemistry,
Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
October 2020, Biochemical and biophysical research communications,
Yanyan Zhao, and Wen Shi, and Xiaohua Li, and Huimin Ma
May 2024, Mini reviews in medicinal chemistry,
Copied contents to your clipboard!