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A synthetic IgG-binding domain based on staphylococcal protein A was designed with the aid of sequence comparisons and computer graphic analysis. A strategy, utilizing non-palindromic restriction sites, was used to overcome the difficulties of introducing site-specific changes into the repetitive gene. A single mutagenized gene fragment was polymerized to different multiplicities, and the different gene products were expressed in Escherichia coli. Using this scheme, protein A-like proteins composed of different numbers of IgG-binding domains were produced. These domains were changed to lack asparagine--glycine dipeptide sequences as well as methionine residues and are thus, in contrast to native protein A, resistant to treatment with hydroxylamine and cyanogen bromide.
B Nilsson, and
T Moks, and
B Jansson, and
L Abrahmsén, and
A Elmblad, and
E Holmgren, and
C Henrichson, and
T A Jones, and
M Uhlén
August 1998,
Molecular biotechnology,
B Nilsson, and
T Moks, and
B Jansson, and
L Abrahmsén, and
A Elmblad, and
E Holmgren, and
C Henrichson, and
T A Jones, and
M Uhlén
January 2014,
Photochemistry and photobiology,
B Nilsson, and
T Moks, and
B Jansson, and
L Abrahmsén, and
A Elmblad, and
E Holmgren, and
C Henrichson, and
T A Jones, and
M Uhlén
March 1989,
Protein engineering,
B Nilsson, and
T Moks, and
B Jansson, and
L Abrahmsén, and
A Elmblad, and
E Holmgren, and
C Henrichson, and
T A Jones, and
M Uhlén
October 2006,
Biochemical and biophysical research communications,
B Nilsson, and
T Moks, and
B Jansson, and
L Abrahmsén, and
A Elmblad, and
E Holmgren, and
C Henrichson, and
T A Jones, and
M Uhlén
November 1997,
Veterinary immunology and immunopathology,
B Nilsson, and
T Moks, and
B Jansson, and
L Abrahmsén, and
A Elmblad, and
E Holmgren, and
C Henrichson, and
T A Jones, and
M Uhlén
March 1988,
The Journal of biological chemistry,
B Nilsson, and
T Moks, and
B Jansson, and
L Abrahmsén, and
A Elmblad, and
E Holmgren, and
C Henrichson, and
T A Jones, and
M Uhlén
December 1983,
FEBS letters,
B Nilsson, and
T Moks, and
B Jansson, and
L Abrahmsén, and
A Elmblad, and
E Holmgren, and
C Henrichson, and
T A Jones, and
M Uhlén
January 1989,
Journal of immunology (Baltimore, Md. : 1950),
B Nilsson, and
T Moks, and
B Jansson, and
L Abrahmsén, and
A Elmblad, and
E Holmgren, and
C Henrichson, and
T A Jones, and
M Uhlén
October 1997,
Biochemical and molecular medicine,
B Nilsson, and
T Moks, and
B Jansson, and
L Abrahmsén, and
A Elmblad, and
E Holmgren, and
C Henrichson, and
T A Jones, and
M Uhlén
March 2022,
Analytical and bioanalytical chemistry,
