Biomaterial Database

Protective effects and regulatory mechanisms of melatonin in a neonatal mouse model of LPS-induced inflammation. 2022

Xinzhu Liu, and Suyan Yao, and Jing Bi, and Deyu Zheng, and Pan Wang

College of Basic Medicine, Jinzhou Medical University, Jinzhou 121000, China.

This experiment mainly explored the protective effect and regulatory mechanism of melatonin (MEL) through its receptor on central nervous system (CNS) inflammation induced by lipopolysaccharide (LPS). The experiment was first divided into the following four groups: control group (CTRL group), LPS-induced inflammation model group (LPS group), LPS-treated MEL group (LPS + MEL group), and MEL administration group (MEL group). Later, a luzindole-antagonized LPS-MEL cotreatment group (LPS + MEL + LUZ group) was added to clarify the experimental results. ELISA was used to determine the inflammatory factor levels IL-6, IL-1β, and IL-10 in brain slices. Western blotting was used to determine the expression levels of the microglia-specific protein CD11b and melatonin receptors MT1 and MT2 in brain slices. A large amount of IL-6 release and increased expression of CD11b protein were detected 24 h after inflammatory stimulation, while pretreatment with MEL inhibited the release of IL-6 and increased the expression of CD11b. At the same time, LPS induction downregulated the relative protein expression levels of MT1 and MT2. In addition, compared with the CTRL group and the LPS + MEL group, the administration of LUZ inhibited the protein expression of MT1. It increased the release of IL-1β and IL-10, further indicating that MEL can alleviate LPS-induced neuroinflammation through the MT1 response. In short, MEL can reduce the neuroinflammatory response induced by LPS and exhibit related protective effects through MT1.

UI	MeSH Term	Description	Entries
D007378	Interleukins	Soluble factors which stimulate growth-related activities of leukocytes as well as other cell types. They enhance cell proliferation and differentiation, DNA synthesis, secretion of other biologically active molecules and responses to immune and inflammatory stimuli.	Interleukin
D008070	Lipopolysaccharides	Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)	Lipopolysaccharide,Lipoglycans
D008550	Melatonin	A biogenic amine that is found in animals and plants. In mammals, melatonin is produced by the PINEAL GLAND. Its secretion increases in darkness and decreases during exposure to light. Melatonin is implicated in the regulation of SLEEP, mood, and REPRODUCTION. Melatonin is also an effective antioxidant.
D001921	Brain	The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.	Encephalon
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000893	Anti-Inflammatory Agents	Substances that reduce or suppress INFLAMMATION.	Anti-Inflammatory Agent,Antiinflammatory Agent,Agents, Anti-Inflammatory,Agents, Antiinflammatory,Anti-Inflammatories,Antiinflammatories,Antiinflammatory Agents,Agent, Anti-Inflammatory,Agent, Antiinflammatory,Agents, Anti Inflammatory,Anti Inflammatories,Anti Inflammatory Agent,Anti Inflammatory Agents
D044094	Receptors, Melatonin	A family of G-protein-coupled receptors that are specific for and mediate the effects of MELATONIN. Activation of melatonin receptors has been associated with decreased intracellular CYCLIC AMP and increased hydrolysis of PHOSPHOINOSITIDES.	Melatonin Receptors,MT3 Receptor,Mel(1c) Receptor,Melatonin MT3 Receptor,Receptor, Mel1c,Receptor, Melatonin,Receptor, Melatonin, 1C,Receptor, Melatonin, MT3,MT3 Receptor, Melatonin,Mel1c Receptor,Melatonin Receptor,Receptor, MT3,Receptor, Melatonin MT3
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D018696	Neuroprotective Agents	Drugs intended to prevent damage to the brain or spinal cord from ischemia, stroke, convulsions, or trauma. Some must be administered before the event, but others may be effective for some time after. They act by a variety of mechanisms, but often directly or indirectly minimize the damage produced by endogenous excitatory amino acids.	Neuroprotectant,Neuroprotective Agent,Neuroprotective Drug,Neuroprotectants,Neuroprotective Drugs,Neuroprotective Effect,Neuroprotective Effects,Agent, Neuroprotective,Agents, Neuroprotective,Drug, Neuroprotective,Drugs, Neuroprotective,Effect, Neuroprotective,Effects, Neuroprotective