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We examined the possibility that mice could be used in the Sereny test instead of guinea pigs or rabbits. Although the reactions in mice were more transient and not as pronounced as those in guinea pigs, mice indeed could be used to distinguish even macroscopically between virulent and avirulent shigellae. Virulent enteroinvasive Escherichia coli strains were also positive for the mouse Sereny test. We described the macroscopic and microscopic appearance of the mouse eyes. Thus, mice are recommended for use in the Sereny test, particularly when a large number of samples are to be tested.
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