Coxiella Burnetii DNA in Milk, Milk Products, and Fermented Dairy Products. 2021

Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, Jelgava, LV-3004, Latvia.

BACKGROUND Q fever in dairy cattle has been investigated in Latvia since 2012. In 2015, 10.7% of farms tested positive for the DNA of C. burnetii, its aetiological agent, in bulk tank milk. The presence of C. burnetii DNA and infectious bacteria in dairy products has been assessed in several countries, and because Latvian milk may contain them, parallel assessment in this country is recommended. Accordingly, the present study tested shop and farm retail dairy products from Latvia and included foreign products for comparison. METHODS Investigation was carried out of 187 samples of a diverse range of dairy products from 41 Latvian milk producers. Twenty-six comparable samples pooled from Estonia, France, Germany, Greece, Italy, Lithuania, the Netherlands, Poland and Spain were also included. The all-countries total number of fermented milk products was 160. Special attention was paid to products that could be more attractive to children because of their added chocolate, cacao, berry and fruit content. DNA was extracted and amplification of C. burnetii IS1111 was performed using a commercial PCR kit. RESULTS Overall positivity was 60.56%. Domestic products were positive more often (60.96%) than foreign ones (57.69%). Only 26.67% of unpasteurised Latvian cow's milk samples were positive whereas 76.47% of pasteurised equivalents and 63.13% of fermented milk products were. Sweetened and fruit-containing samples were 71.43% positive. CONCLUSIONS The shedding of C. burnetii via milk should be monitored and only milk from healthy animals allowed for sale for direct human consumption without pasteurisation. Raw milk quality and the effectiveness of industrial heat treatment and pasteurisation methods in Latvia and other countries should be carefully assessed to ensure adequate consumer health protection.

UI MeSH Term Description Entries

Related Publications

Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
April 2013, The American journal of tropical medicine and hygiene,
Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
April 2022, International journal of food microbiology,
Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
January 2008, Veterinary research,
Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
March 1985, International journal of zoonoses,
Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
August 2018, Iranian journal of microbiology,
Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
January 2007, Veterinary research,
Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
August 2012, The Veterinary record,
Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
December 2007, Journal of dairy science,
Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
March 2011, Vaccine,
Linda Valkovska, and Artjoms Mališevs, and Kaspars Kovaļenko, and Aivars Bērziņš, and Lelde Grantiņa-Ieviņa
March 2021, Pathogens (Basel, Switzerland),
Copied contents to your clipboard!