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Cloned cDNAs have been isolated that encode a variant of hirudin, a potent thrombin inhibitor that is secreted by the salivary glands of the medicinal leech, Hirudo medicinalis. This variant probably corresponds to a form that has been purified from leech heads but differs in amino acid sequence from the hirudin purified from whole leeches. There are at least three hirudin transcripts detectable in leech RNAs that are different in size, site of synthesis, inducibility by starvation, and relationship to hirudin activity. The new hirudin variant predicted by the cDNA and the heterodisperse transcription products suggest a hirudin protein family. The hirudin cDNA was expressed in Escherichia coli under the control of the bacteriophage lambda PL promoter. The recombinant product is biologically active, inhibiting the cleavage by thrombin of fibrinogen and a synthetic tripeptide substrate.
R P Harvey, and
E Degryse, and
L Stefani, and
F Schamber, and
J P Cazenave, and
M Courtney, and
P Tolstoshev, and
J P Lecocq
August 1986,
Biological chemistry Hoppe-Seyler,
R P Harvey, and
E Degryse, and
L Stefani, and
F Schamber, and
J P Cazenave, and
M Courtney, and
P Tolstoshev, and
J P Lecocq
December 1998,
The Journal of biological chemistry,
R P Harvey, and
E Degryse, and
L Stefani, and
F Schamber, and
J P Cazenave, and
M Courtney, and
P Tolstoshev, and
J P Lecocq
December 1999,
Biulleten' eksperimental'noi biologii i meditsiny,
R P Harvey, and
E Degryse, and
L Stefani, and
F Schamber, and
J P Cazenave, and
M Courtney, and
P Tolstoshev, and
J P Lecocq
January 1992,
Duodecim; laaketieteellinen aikakauskirja,
R P Harvey, and
E Degryse, and
L Stefani, and
F Schamber, and
J P Cazenave, and
M Courtney, and
P Tolstoshev, and
J P Lecocq
February 1999,
Gene,
R P Harvey, and
E Degryse, and
L Stefani, and
F Schamber, and
J P Cazenave, and
M Courtney, and
P Tolstoshev, and
J P Lecocq
September 2001,
The Journal of audiovisual media in medicine,
R P Harvey, and
E Degryse, and
L Stefani, and
F Schamber, and
J P Cazenave, and
M Courtney, and
P Tolstoshev, and
J P Lecocq
December 1987,
Tijdschrift voor ziekenverpleging,
R P Harvey, and
E Degryse, and
L Stefani, and
F Schamber, and
J P Cazenave, and
M Courtney, and
P Tolstoshev, and
J P Lecocq
April 1991,
Seminars in thrombosis and hemostasis,
R P Harvey, and
E Degryse, and
L Stefani, and
F Schamber, and
J P Cazenave, and
M Courtney, and
P Tolstoshev, and
J P Lecocq
January 1990,
Journal de pharmacie de Belgique,
R P Harvey, and
E Degryse, and
L Stefani, and
F Schamber, and
J P Cazenave, and
M Courtney, and
P Tolstoshev, and
J P Lecocq
July 2001,
Biochemistry. Biokhimiia,
