BIOMAT Database Logo BIOMAT Database Logo

Replacement of wybutine by hydrazines and its effect on the active conformation of yeast tRNAPhe. 1978

H G Schleich, and W Wintermeyer, and H G Zachau

The highly modified base wybutine (YWye) next to the anticodon of yeast tRNAPhe has been replaced by different hydrazine derivatives. The effect of the replacement on the activity of the tRNA has been studied in the heterologous aminoacylation with synthetase from E. coli and in the poly(U) directed binding to ribosomes from both yeast and E. coli. It was found that starting from tRNA-PheYWye the activity increased with increasing size, aromaticity, and stacking tendency of the substituent replacing YWye. It is concluded that YWye by the size of its aromatic system and by its stacking properties is particularly well suited for stabilizing the native conformation of tRNAPhe.

UI MeSH Term Description Entries
D007700 Kinetics The rate dynamics in chemical or physical systems.
D010649 Phenylalanine An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE. Endorphenyl,L-Phenylalanine,Phenylalanine, L-Isomer,L-Isomer Phenylalanine,Phenylalanine, L Isomer
D010652 Phenylalanine-tRNA Ligase An enzyme that activates phenylalanine with its specific transfer RNA. EC 6.1.1.20. Phenylalanyl T RNA Synthetase,Phe-tRNA Ligase,Phenylalanyl-tRNA Synthetase,Ligase, Phe-tRNA,Ligase, Phenylalanine-tRNA,Phe tRNA Ligase,Phenylalanine tRNA Ligase,Phenylalanyl tRNA Synthetase,Synthetase, Phenylalanyl-tRNA
D011072 Poly U A group of uridine ribonucleotides in which the phosphate residues of each uridine ribonucleotide act as bridges in forming diester linkages between the ribose moieties. Polyuridylic Acids,Uracil Polynucleotides,Poly(rU),Acids, Polyuridylic,Polynucleotides, Uracil
D011688 Purinones Oxopurines
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006147 Guanine
D006834 Hydrazines Substituted derivatives of hydrazine (formula H2N-NH2). Hydrazide
D012270 Ribosomes Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION. Ribosome
D012343 RNA, Transfer The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains. Suppressor Transfer RNA,Transfer RNA,tRNA,RNA, Transfer, Suppressor,Transfer RNA, Suppressor,RNA, Suppressor Transfer

Related Publications

H G Schleich, and W Wintermeyer, and H G Zachau
Conformational dynamics of the anticodon loop in yeast tRNAPhe as sensed by the fluorescence of wybutine.
January 1986, European biophysics journal : EBJ,
H G Schleich, and W Wintermeyer, and H G Zachau
Effect of ribosome binding and translocation on the anticodon of tRNAPhe as studied by wybutine fluorescence.
April 1982, Nucleic acids research,
H G Schleich, and W Wintermeyer, and H G Zachau
The conformation of the anticodon loop of yeast tRNAPhe in solution and on ribosomes.
December 1978, Biopolymers,
H G Schleich, and W Wintermeyer, and H G Zachau
Effect of elongation factor Tu on the conformation of phenylalanyl-tRNAPhe.
April 1983, FEBS letters,
H G Schleich, and W Wintermeyer, and H G Zachau
Effect of magnesium and polyamines on the structure of yeast tRNAPhe.
July 1977, Biochimica et biophysica acta,
H G Schleich, and W Wintermeyer, and H G Zachau
Yeast tRNAPhe conformation wheels: a novel probe of the monoclinic and orthorhombic models.
May 1980, Nucleic acids research,
H G Schleich, and W Wintermeyer, and H G Zachau
The effect of chemical modification of the CCA end of yeast tRNAPhe on its biological activity on ribosomes.
January 1980, European journal of biochemistry,
H G Schleich, and W Wintermeyer, and H G Zachau
The conformation of the tRNAPhe anticodon loop monitored by fluorescence.
February 1984, Nucleic acids research,
H G Schleich, and W Wintermeyer, and H G Zachau
Effect of translocation on topology and conformation of anticodon and D loops of tRNAPhe.
September 1981, Journal of molecular biology,
H G Schleich, and W Wintermeyer, and H G Zachau
Procedure for C2 deuteration of nucleic acids and determination of A psi 31 pseudouridine conformation by nuclear Overhauser effect in yeast tRNAPhe.
December 1982, Nucleic acids research,
Copied contents to your clipboard!