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A sensitive two-site enzyme immunoassay (EIA) for human epidermal growth factor (hEGF) was developed, based on the sandwiching of an antigen between anti-hEGF IgG-coated polystyrene beads and anti-hEGF Fab'-linked peroxidase complex (horseradish peroxidase, EC. 1.11.1.7). This method has four advantages: the anti-hEGF Fab'-linked peroxidase complex is more stable than 125I-labelled antibody; the procedure is simple and rapid compared to bioassay; its discriminatory sensitivity is as low as 0.1 pg/assay tube; and serial dilution curves of unextracted human serum and urine samples all paralleled that of standard hEGF. The validity of the measurement of hEGF-like immunoreactivity in human serum and plasma is discussed.
M Kurobe, and
N Tokida, and
S Furukawa, and
E Ishikawa, and
K Hayashi
June 1985,
Biochemistry international,
M Kurobe, and
N Tokida, and
S Furukawa, and
E Ishikawa, and
K Hayashi
January 1987,
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology,
M Kurobe, and
N Tokida, and
S Furukawa, and
E Ishikawa, and
K Hayashi
September 1991,
Biochemistry international,
M Kurobe, and
N Tokida, and
S Furukawa, and
E Ishikawa, and
K Hayashi
September 1987,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
M Kurobe, and
N Tokida, and
S Furukawa, and
E Ishikawa, and
K Hayashi
June 1978,
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M Kurobe, and
N Tokida, and
S Furukawa, and
E Ishikawa, and
K Hayashi
December 1977,
The Journal of clinical endocrinology and metabolism,
M Kurobe, and
N Tokida, and
S Furukawa, and
E Ishikawa, and
K Hayashi
February 1991,
Biochemical and biophysical research communications,
M Kurobe, and
N Tokida, and
S Furukawa, and
E Ishikawa, and
K Hayashi
April 1984,
Sheng li ke xue jin zhan [Progress in physiology],
M Kurobe, and
N Tokida, and
S Furukawa, and
E Ishikawa, and
K Hayashi
April 1979,
The Journal of clinical endocrinology and metabolism,
M Kurobe, and
N Tokida, and
S Furukawa, and
E Ishikawa, and
K Hayashi
January 2001,
Human antibodies,
