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The ability to measure cell proliferation is important in the study of cancer biology. The usual technique for quantitating proliferating cells in tissue explant and organ culture by detection of [3H]-thymidine incorporation into DNA by autoradiography is tedious and time-consuming. We have developed a technique for identification and quantitation of bromodeoxyuridine (an analogue of thymidine) in cultured tissue explants. Fetal mouse colon explants were exposed in vitro to bromodeoxyuridine (BUdR) or [3H]-thymidine for 3 to 72 hr and then for various periods to unlabeled thymidine. The tissues were stained with a monoclonal anti-bromodeoxyuridine antibody and in parallel [3H]-thymidine incorporation was detected by autoradiography. Incorporation of BUdR was measured by quantitating the amount of pigment deposited over nuclei after immunohistochemical staining, using an optical data digitizer. It was found that both techniques identified proliferating cells. Dividing cells were present both in crypts and in the surrounding stroma in Day 14 fetal mouse colon cultures. The immunohistochemical technique was more rapid and less cumbersome than autoradiography.
G Morstyn, and
K Pyke, and
J Gardner, and
R Ashcroft, and
A de Fazio, and
P Bhathal
October 1991,
Neuroscience letters,
G Morstyn, and
K Pyke, and
J Gardner, and
R Ashcroft, and
A de Fazio, and
P Bhathal
January 1994,
Gynecologic and obstetric investigation,
G Morstyn, and
K Pyke, and
J Gardner, and
R Ashcroft, and
A de Fazio, and
P Bhathal
February 1988,
Neurosurgery,
G Morstyn, and
K Pyke, and
J Gardner, and
R Ashcroft, and
A de Fazio, and
P Bhathal
December 1989,
Journal of Korean medical science,
G Morstyn, and
K Pyke, and
J Gardner, and
R Ashcroft, and
A de Fazio, and
P Bhathal
January 1986,
Basic and applied histochemistry,
G Morstyn, and
K Pyke, and
J Gardner, and
R Ashcroft, and
A de Fazio, and
P Bhathal
April 1996,
Journal of comparative pathology,
G Morstyn, and
K Pyke, and
J Gardner, and
R Ashcroft, and
A de Fazio, and
P Bhathal
September 2003,
Histochemistry and cell biology,
G Morstyn, and
K Pyke, and
J Gardner, and
R Ashcroft, and
A de Fazio, and
P Bhathal
June 1986,
Japanese journal of clinical oncology,
G Morstyn, and
K Pyke, and
J Gardner, and
R Ashcroft, and
A de Fazio, and
P Bhathal
March 1988,
Nihon Hinyokika Gakkai zasshi. The japanese journal of urology,
G Morstyn, and
K Pyke, and
J Gardner, and
R Ashcroft, and
A de Fazio, and
P Bhathal
February 1994,
Cancer,
