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The rat 3-methylcholanthrene-inducible family of liver cytochromes P-450 contains two proteins (P-450c and P-450d) that are immunochemically related, possess 68% total sequence homology, and are induced by a number of toxic or carcinogenic compounds. To determine whether equivalent isozymes of hepatic cytochrome P-450 are expressed in humans, as they are in several mammalian species, we performed immunoblot analyses on microsomes prepared from 14 human liver specimens and found that each one contained a 52.5-kDa protein (termed HLd) that reacted with antibodies specific for rat P-450d. In addition, one specimen contained a 54-kDa protein (termed HLc) that reacted with antibodies specific for rat P-450c. HLd was purified through the use of immunoaffinity chromatography and was found to be 56% homologous to rat P-450d and 61% homologous to the equivalent isozyme in the rabbit (P-450 LM4) through their first 18 NH2-terminal amino acids. Finally, levels of immunoreactive HLd varied more than 10-fold among these patients but were unrelated to the patients' drug treatments, smoking habits, or amount of immunoreactive HLp, a human liver cytochrome P-450 related to the glucocorticoid-inducible family of rat cytochromes P-450. We conclude that, in man, there is a cytochrome P-450 family composed of two isozymes (HLc and HLd) that are immunochemically and structurally related to the 3-methylcholanthrene-inducible family observed in several other species.
S A Wrighton, and
C Campanile, and
P E Thomas, and
S L Maines, and
P B Watkins, and
G Parker, and
G Mendez-Picon, and
M Haniu, and
J E Shively, and
W Levin
January 1989,
Pharmacology,
S A Wrighton, and
C Campanile, and
P E Thomas, and
S L Maines, and
P B Watkins, and
G Parker, and
G Mendez-Picon, and
M Haniu, and
J E Shively, and
W Levin
September 1985,
Proceedings of the National Academy of Sciences of the United States of America,
S A Wrighton, and
C Campanile, and
P E Thomas, and
S L Maines, and
P B Watkins, and
G Parker, and
G Mendez-Picon, and
M Haniu, and
J E Shively, and
W Levin
August 1988,
Biochemical pharmacology,
S A Wrighton, and
C Campanile, and
P E Thomas, and
S L Maines, and
P B Watkins, and
G Parker, and
G Mendez-Picon, and
M Haniu, and
J E Shively, and
W Levin
July 1985,
The Journal of biological chemistry,
S A Wrighton, and
C Campanile, and
P E Thomas, and
S L Maines, and
P B Watkins, and
G Parker, and
G Mendez-Picon, and
M Haniu, and
J E Shively, and
W Levin
July 1988,
Archives of biochemistry and biophysics,
S A Wrighton, and
C Campanile, and
P E Thomas, and
S L Maines, and
P B Watkins, and
G Parker, and
G Mendez-Picon, and
M Haniu, and
J E Shively, and
W Levin
July 1983,
Proceedings of the National Academy of Sciences of the United States of America,
S A Wrighton, and
C Campanile, and
P E Thomas, and
S L Maines, and
P B Watkins, and
G Parker, and
G Mendez-Picon, and
M Haniu, and
J E Shively, and
W Levin
October 1985,
DNA (Mary Ann Liebert, Inc.),
S A Wrighton, and
C Campanile, and
P E Thomas, and
S L Maines, and
P B Watkins, and
G Parker, and
G Mendez-Picon, and
M Haniu, and
J E Shively, and
W Levin
September 1987,
Biochemical pharmacology,
S A Wrighton, and
C Campanile, and
P E Thomas, and
S L Maines, and
P B Watkins, and
G Parker, and
G Mendez-Picon, and
M Haniu, and
J E Shively, and
W Levin
May 1982,
Proceedings of the National Academy of Sciences of the United States of America,
S A Wrighton, and
C Campanile, and
P E Thomas, and
S L Maines, and
P B Watkins, and
G Parker, and
G Mendez-Picon, and
M Haniu, and
J E Shively, and
W Levin
November 1988,
Biochemical and biophysical research communications,
