BIOMAT Database Logo BIOMAT Database Logo

The structure of cat muscle pyruvate kinase. 1986

H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt

The complete amino acid sequence of cat muscle pyruvate kinase has been determined and fitted to the 2.6 A resolution electron density map. Residues in the active site region are highly conserved in the cat muscle, chicken muscle, rat liver and yeast enzymes. The enzyme-bound magnesium, which is essential for activity, interacts with the side chain of glutamate-271 and with two main carbonyl groups. Lysine-269 is the probable acid/base catalyst responsible for the interconversion of pyruvate and enolpyruvate. A possible binding site for the essential monovalent cation is proposed.

UI MeSH Term Description Entries
D008958 Models, Molecular Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures. Molecular Models,Model, Molecular,Molecular Model
D009132 Muscles Contractile tissue that produces movement in animals. Muscle Tissue,Muscle,Muscle Tissues,Tissue, Muscle,Tissues, Muscle
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D011770 Pyruvate Kinase ATP:pyruvate 2-O-phosphotransferase. A phosphotransferase that catalyzes reversibly the phosphorylation of pyruvate to phosphoenolpyruvate in the presence of ATP. It has four isozymes (L, R, M1, and M2). Deficiency of the enzyme results in hemolytic anemia. EC 2.7.1.40. L-Type Pyruvate Kinase,M-Type Pyruvate Kinase,M1-Type Pyruvate Kinase,M2-Type Pyruvate Kinase,Pyruvate Kinase L,R-Type Pyruvate Kinase,L Type Pyruvate Kinase,M Type Pyruvate Kinase,M1 Type Pyruvate Kinase,M2 Type Pyruvate Kinase,Pyruvate Kinase, L-Type,Pyruvate Kinase, M-Type,Pyruvate Kinase, M1-Type,Pyruvate Kinase, M2-Type,Pyruvate Kinase, R-Type,R Type Pyruvate Kinase
D002415 Cats The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801) Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001665 Binding Sites The parts of a macromolecule that directly participate in its specific combination with another molecule. Combining Site,Binding Site,Combining Sites,Site, Binding,Site, Combining,Sites, Binding,Sites, Combining
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker

Related Publications

H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt
Structure of cat muscle pyruvate kinase at 0.26 nm resolution.
January 1977, Biochemical Society transactions,
H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt
Three-dimensional structure of cat muscle pyruvate kinase at 6 Angstrom resolution.
June 1975, Journal of molecular biology,
H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt
Crystal structure of cat muscle pyruvate kinase at a resolution of 2.6 A.
October 1979, Journal of molecular biology,
H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt
Three-dimensional structure of cat muscle pyruvate kinase at 3-1 A resolution.
May 1977, Journal of molecular biology,
H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt
Subunit structure of rabbit muscle pyruvate kinase.
March 1969, The Journal of biological chemistry,
H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt
Primary structure of chicken muscle pyruvate kinase mRNA.
June 1983, Proceedings of the National Academy of Sciences of the United States of America,
H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt
Refined three-dimensional structure of cat-muscle (M1) pyruvate kinase at a resolution of 2.6 A.
May 1996, Acta crystallographica. Section D, Biological crystallography,
H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt
Structure of rabbit muscle pyruvate kinase complexed with Mn2+, K+, and pyruvate.
May 1994, Biochemistry,
H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt
Cat muscle pyruvate kinase: isolation of a reactive thiol-group-containing peptide [proceedings].
January 1977, Biochemical Society transactions,
H Muirhead, and D A Clayden, and D Barford, and C G Lorimer, and L A Fothergill-Gilmore, and E Schiltz, and W Schmitt
The subunit structure of human muscle and human erythrocyte pyruvate kinase isozymes.
December 1974, FEBS letters,
Copied contents to your clipboard!