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An in vitro penetration test using human spermatozoa, sera, and eggs stored in a highly concentrated salt solution was designed for examination of the effect of antisperm antibodies on the process of fertilization. Spermatozoa from a healthy fertile donor incubated in modified Biggers, Whiiten and Whittingham (BWW) medium containing 7.5% antisperm-antibody-negative serum, could penetrate through the zonae pellucidae of the stored eggs, but not when the spermatozoa from the same donor had been incubated in modified BWW medium containing 7.5% antisperm-antibody-positive serum. After the antisperm-antibody-positive serum was absorbed with washed spermatozoa, the sperm penetration was not blocked. Therefore, antisperm antibodies appear to block human sperm penetration through the human zona pellucida.
S Tsukui, and
Y Noda, and
J Yano, and
A Fukuda, and
T Mori
February 1991,
Fertility and sterility,
S Tsukui, and
Y Noda, and
J Yano, and
A Fukuda, and
T Mori
August 1985,
Journal of reproductive immunology,
S Tsukui, and
Y Noda, and
J Yano, and
A Fukuda, and
T Mori
January 2008,
The International journal of developmental biology,
S Tsukui, and
Y Noda, and
J Yano, and
A Fukuda, and
T Mori
September 1985,
Biology of reproduction,
S Tsukui, and
Y Noda, and
J Yano, and
A Fukuda, and
T Mori
October 1962,
Journal of reproduction and fertility,
S Tsukui, and
Y Noda, and
J Yano, and
A Fukuda, and
T Mori
January 1974,
Acta physiologica latino americana,
S Tsukui, and
Y Noda, and
J Yano, and
A Fukuda, and
T Mori
March 1965,
The Journal of experimental zoology,
S Tsukui, and
Y Noda, and
J Yano, and
A Fukuda, and
T Mori
January 1981,
Progress in clinical and biological research,
S Tsukui, and
Y Noda, and
J Yano, and
A Fukuda, and
T Mori
February 1990,
The Journal of reproductive medicine,
S Tsukui, and
Y Noda, and
J Yano, and
A Fukuda, and
T Mori
May 1999,
Andrologia,
