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A human tumor cell line (EJ) expressing an activated c-Ha-ras oncogene was fused with a normal human fibroblast cell line. This fusion resulted in hybrids that behaved as transformed cells in culture but failed to form tumors in nude (athymic) mice. After repeated cell passage, two tumorigenic segregants of the hybrids arose in culture. The levels of expression of activated c-Ha-ras mRNA and its protein product, p21, were similar in the EJ cell line, the nontumorigenic hybrids, and the tumorigenic segregants. DNA transfections of the hybrids were performed with activated c-Ha-ras plasmid constructs, and transfectants expressing a 2-fold level of c-Ha-ras relative to the hybrid cells were found to maintain the nontumorigenic phenotype. We suggest that expression of the active c-Ha-ras oncogene is insufficient for the malignant transformation of these human cells.
A G Geiser, and
C J Der, and
C J Marshall, and
E J Stanbridge
January 1983,
Nature,
A G Geiser, and
C J Der, and
C J Marshall, and
E J Stanbridge
June 1989,
Journal of the National Cancer Institute,
A G Geiser, and
C J Der, and
C J Marshall, and
E J Stanbridge
June 1990,
Cell biology international reports,
A G Geiser, and
C J Der, and
C J Marshall, and
E J Stanbridge
January 1993,
In vivo (Athens, Greece),
A G Geiser, and
C J Der, and
C J Marshall, and
E J Stanbridge
June 1982,
Nature,
A G Geiser, and
C J Der, and
C J Marshall, and
E J Stanbridge
July 1987,
Bioscience reports,
A G Geiser, and
C J Der, and
C J Marshall, and
E J Stanbridge
January 1991,
Molecular carcinogenesis,
A G Geiser, and
C J Der, and
C J Marshall, and
E J Stanbridge
March 1986,
Cancer research,
A G Geiser, and
C J Der, and
C J Marshall, and
E J Stanbridge
May 1989,
International journal of cancer,
A G Geiser, and
C J Der, and
C J Marshall, and
E J Stanbridge
September 1987,
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology,
