BIOMAT Database Logo BIOMAT Database Logo

Studies of virus structure by laser-Raman spectroscopy. Turnip yellow mosaic virus and capsids. 1978

K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas

Laser-Raman spectroscopy of the turnip yellow mosaic virus (TYMV) and its capsid indicate the following features of the structure and assembly of the virion. The secondary structure of coat-protein molecules in TYMV is comprised of 9 +/- 5% alpha-helix, 43 +/- 6% beta-sheet, and 48 +/- 6% irregular conformation and is not altered by the removal of the RNA from the capsid. Introduction of as many as 200 chain scissions per RNA molecule also does not affect the overall secondary structure of the encapsulated RNA, which is 77 +/- 5% in the A-helix form. Tryptophan and cysteine residues of the coat protein appear to be in contact with the solvent, while only one of three tyrosines per coat protein is available for hydrogen bonding of its p-hydroxyl group with H2O molecules. Both cytosine and adenine residues of TYMV RNA are protonated in substantial numbers near pH 4.5, suggesting elevation of their respective pKa values within the virion. The Raman data are consistent with chemical evidence favoring interaction between protonated bases of RNA and amino acid side chains of coat protein in TYMV.

UI MeSH Term Description Entries
D007834 Lasers An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum. Masers,Continuous Wave Lasers,Pulsed Lasers,Q-Switched Lasers,Continuous Wave Laser,Laser,Laser, Continuous Wave,Laser, Pulsed,Laser, Q-Switched,Lasers, Continuous Wave,Lasers, Pulsed,Lasers, Q-Switched,Maser,Pulsed Laser,Q Switched Lasers,Q-Switched Laser
D009029 Mosaic Viruses Viruses which produce a mottled appearance of the leaves of plants. Mosaic Virus,Virus, Mosaic,Viruses, Mosaic
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D010942 Plant Viruses Viruses parasitic on plants. Phytophagineae,Plant Virus,Virus, Plant,Viruses, Plant
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D002213 Capsid The outer protein protective shell of a virus, which protects the viral nucleic acid. Capsids are composed of repeating units (capsomers or capsomeres) of CAPSID PROTEINS which when assembled together form either an icosahedral or helical shape. Procapsid,Prohead,Capsids,Procapsids,Proheads
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D012335 RNA, Ribosomal The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed) Ribosomal RNA,15S RNA,RNA, 15S
D013059 Spectrum Analysis, Raman Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light. Raman Spectroscopy,Analysis, Raman Spectrum,Raman Optical Activity Spectroscopy,Raman Scattering,Raman Spectrum Analysis,Scattering, Raman,Spectroscopy, Raman
D014764 Viral Proteins Proteins found in any species of virus. Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral

Related Publications

K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas
Structure of turnip yellow mosaic virus.
March 1957, Nature,
K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas
Crystal structure of turnip yellow mosaic virus.
September 1996, Nature structural biology,
K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas
The structure of turnip yellow mosaic virus; x-ray diffraction studies.
August 1957, Biochimica et biophysica acta,
K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas
Turnip yellow mosaic virus.
January 1966, Advances in virus research,
K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas
Chemical accessibility of tyrosyl and lysyl residues in turnip yellow mosaic virus capsids.
October 1975, Biochemistry,
K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas
Empty Turnip yellow mosaic virus capsids as delivery vehicles to mammalian cells.
July 2018, Virus research,
K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas
Studies on the virus of turnip yellow mosaic.
February 1949, Parasitology,
K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas
[Action of urea on the turnip yellow mosaic virus: formation of artificial capsids].
December 1966, Comptes rendus hebdomadaires des seances de l'Academie des sciences. Serie D: Sciences naturelles,
K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas
Primary structure of turnip yellow mosaic virus coat protein.
August 1972, Virology,
K A Hartman, and P E McDonald-Ordzie, and J M Kaper, and B Prescott, and G J Thomas
Studies on infectious RNA from turnip yellow mosaic virus.
May 1962, Journal of molecular biology,
Copied contents to your clipboard!