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We have compared a new enzyme immunoassay (EIA) for estrogen receptors (ER) with our conventional radioligand binding assays (multipoint dextran-coated charcoal assay for cytoplasmic ER and hydroxylapatite exchange assay for nuclear ER). Cytoplasmic ERs were measured in 76 human breast cancer specimens by EIA and by five-point Scatchard analysis. The correlation between the two assays yielded a straight line with a slope of 0.92 (r = 0.95; P less than 0.001); conversely, in 31 nuclear salt extracts, linear regression analysis of hydroxylapatite exchange assay data with EIA showed a clear correlation (r = 0.93; P less than 0.001) but a slope of 1.7, demonstrating that EIA detects more ER sites. The binding of the antibody to the cytoplasmic ER molecules was investigated by sucrose density gradient analysis, which showed that EIA recognizes both cytoplasmic forms (9 and 3S), but does not distinguish between them. Advantages and drawbacks of this method are discussed with respect to its application for routine receptor determination for clinical management of breast cancer patients.
G Di Fronzo, and
P Miodini, and
M Brivio, and
V Cappelletti, and
D Coradini, and
G Granata, and
E Ronchi
January 1985,
Cancer research,
G Di Fronzo, and
P Miodini, and
M Brivio, and
V Cappelletti, and
D Coradini, and
G Granata, and
E Ronchi
April 1988,
Tumori,
G Di Fronzo, and
P Miodini, and
M Brivio, and
V Cappelletti, and
D Coradini, and
G Granata, and
E Ronchi
January 1989,
Methods in enzymology,
G Di Fronzo, and
P Miodini, and
M Brivio, and
V Cappelletti, and
D Coradini, and
G Granata, and
E Ronchi
January 1994,
Anticancer research,
G Di Fronzo, and
P Miodini, and
M Brivio, and
V Cappelletti, and
D Coradini, and
G Granata, and
E Ronchi
September 2008,
Journal of neuroscience methods,
G Di Fronzo, and
P Miodini, and
M Brivio, and
V Cappelletti, and
D Coradini, and
G Granata, and
E Ronchi
January 1990,
Methods in enzymology,
G Di Fronzo, and
P Miodini, and
M Brivio, and
V Cappelletti, and
D Coradini, and
G Granata, and
E Ronchi
August 2000,
Steroids,
G Di Fronzo, and
P Miodini, and
M Brivio, and
V Cappelletti, and
D Coradini, and
G Granata, and
E Ronchi
January 2002,
Methods in molecular biology (Clifton, N.J.),
G Di Fronzo, and
P Miodini, and
M Brivio, and
V Cappelletti, and
D Coradini, and
G Granata, and
E Ronchi
January 2016,
Methods in molecular biology (Clifton, N.J.),
G Di Fronzo, and
P Miodini, and
M Brivio, and
V Cappelletti, and
D Coradini, and
G Granata, and
E Ronchi
January 1996,
The International journal of biological markers,
