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The purpose of the present longitudinal investigation was to assess the predictive value of urinary protein analysis in the early detection of rejection crisis after renal transplantation. Forty-one children were studied consecutively over a period of 6 months applying the following methods: creatinine clearance (Ccr); urinary total protein (UTP); and electrophoretic differentiation of urinary proteins according to their molecular size by microgradient-gel electrophoresis (MGGE) with a continuous concentration gradient of 4-40% of polyacrylamide. Protein fractions analyzed were albumin (69,000 d), low molecular weight proteins (LMW-proteins, less than 69,000 d), and high molecular weight proteins (HMW-proteins, greater than 69,000 d). No rejection was observed in 30 children (group A), a total of 18 rejection episodes occurred in 11 children (group B). UTP was significantly lower in group A as compared to group B (107 vs 376 mg/m2/24 h), but no differences in urinary protein pattern were observed between group A and group B prior to rejection. One to two days after rejection UTP increased to 938 mg/m2/24 h, and 3-7 days after rejection LMW-protein fraction increased from 9% to 23% with a corresponding decrease of albumin fraction from 71% to 56% of UTP. No qualitative changes were noted in respect to HMW-protein excretion. It is concluded that changes of UTP and urinary protein pattern occur during rejection episodes but are of no predictive value in detecting rejection before clinical symptoms appear.
R Burghard, and
R G Galaske, and
G Offner, and
J H Ehrich, and
J U Leititis, and
J Brodehl
February 1983,
Clinical nephrology,
R Burghard, and
R G Galaske, and
G Offner, and
J H Ehrich, and
J U Leititis, and
J Brodehl
January 2003,
Transplantation,
R Burghard, and
R G Galaske, and
G Offner, and
J H Ehrich, and
J U Leititis, and
J Brodehl
July 1987,
Pediatric nephrology (Berlin, Germany),
R Burghard, and
R G Galaske, and
G Offner, and
J H Ehrich, and
J U Leititis, and
J Brodehl
April 1976,
Clinical nephrology,
R Burghard, and
R G Galaske, and
G Offner, and
J H Ehrich, and
J U Leititis, and
J Brodehl
March 2001,
Transplantation,
R Burghard, and
R G Galaske, and
G Offner, and
J H Ehrich, and
J U Leititis, and
J Brodehl
January 1976,
Wiener klinische Wochenschrift. Supplementum,
R Burghard, and
R G Galaske, and
G Offner, and
J H Ehrich, and
J U Leititis, and
J Brodehl
May 2009,
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia,
R Burghard, and
R G Galaske, and
G Offner, and
J H Ehrich, and
J U Leititis, and
J Brodehl
August 1998,
Transplantation,
R Burghard, and
R G Galaske, and
G Offner, and
J H Ehrich, and
J U Leititis, and
J Brodehl
January 1992,
Investigacion clinica,
R Burghard, and
R G Galaske, and
G Offner, and
J H Ehrich, and
J U Leititis, and
J Brodehl
January 1997,
Transplantation proceedings,
