BIOMAT Database Logo BIOMAT Database Logo

[Construction and study of a new type of phage lambda DNA vector molecule]. 1978

N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov

A group of lambda mutants (mutants lambda 0) harbouring lesser number of EcoRI restriction sites on DNA molecules was selected. lambda3-1 recombinant (genotype lambdab221amgamma210Sr1lambda3+c-Px) was created by crosses of lambda02 phage with other lambda mutants. This phage DNA may be used as a vector molecule which makes it possible to select easily phages harbouring insertions of EcoRI DNA fragments. The maximal size of DNA fragment, the insertion of which would not decrease lambda3-1 viability, is 7.7 megadaltone. Lambda3-1 DNA has three regions heterological to lambda DNA, two of which probably include sites SRIlambda4 and SRIlambda5 and some juxtaposed genes. For example, Ptgene of lambda phage in juxtaposition with site SRIlambda4 is substituted by Px gene on the lambda3-1 DNA molecule.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D003090 Coliphages Viruses whose host is Escherichia coli. Escherichia coli Phages,Coliphage,Escherichia coli Phage,Phage, Escherichia coli,Phages, Escherichia coli
D003433 Crosses, Genetic Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species. Cross, Genetic,Genetic Cross,Genetic Crosses
D004262 DNA Restriction Enzymes Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1. Restriction Endonucleases,DNA Restriction Enzyme,Restriction Endonuclease,Endonuclease, Restriction,Endonucleases, Restriction,Enzymes, DNA Restriction,Restriction Enzyme, DNA,Restriction Enzymes, DNA
D004274 DNA, Recombinant Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected. Genes, Spliced,Recombinant DNA,Spliced Gene,Recombinant DNA Research,Recombination Joint,DNA Research, Recombinant,Gene, Spliced,Joint, Recombination,Research, Recombinant DNA,Spliced Genes
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D005814 Genes, Viral The functional hereditary units of VIRUSES. Viral Genes,Gene, Viral,Viral Gene
D012641 Selection, Genetic Differential and non-random reproduction of different genotypes, operating to alter the gene frequencies within a population. Natural Selection,Genetic Selection,Selection, Natural

Related Publications

N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov
Construction of an ordered overlapping library of bacteriophage P1 DNA in phage vector lambda D69.
January 1987, Gene,
N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov
[Construction and properties of the expression vector based on the temperature-regulated P'R promoter in phage lambda DNA].
November 1987, Bioorganicheskaia khimiia,
N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov
Improved derivative of a phage lambda EK2 vector for cloning recombinant DNA.
October 1976, Nature,
N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov
Construction and characterization of plasmid and lambda phage vector systems for study of transcriptional control in Escherichia coli.
January 1987, Gene,
N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov
A denaturation map of the lambda phage DNA molecule determined by electron microscopy.
July 1966, Journal of molecular biology,
N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov
Lambda foo: a lambda phage vector for the expression of foreign proteins.
August 1994, Proceedings of the National Academy of Sciences of the United States of America,
N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov
A programme for the construction of a lambda phage.
November 1984, Journal of embryology and experimental morphology,
N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov
A new method for purifying lambda DNA from phage lysates.
February 1985, DNA (Mary Ann Liebert, Inc.),
N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov
Replication of phage lambda DNA.
January 1968, Cold Spring Harbor symposia on quantitative biology,
N K Iankovskii, and B A Rebentish, and V G Bogush, and V N Krylov
Preparation of phage lambda DNA.
January 1985, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!