BIOMAT Database Logo BIOMAT Database Logo

[Purification and properties of Bordetella pertussis toxin]. 1986

A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva

The modified method for the isolation and purification of B. pertussis toxin has been proposed. Chromatography with the use of hydroxylapatite and lentil lectin--Sepharose 4B has permitted the isolation of the preparation purified 600 times. Its molecular weight is about 90,000. The preparation has been found to possess leukocytosis-stimulating, histamine-sensitising and hemagglutinating activity. Electrophoretic analysis has revealed that the isolated substance consists of four subunits with molecular weights 28,400, 24,300, 21,800 and 15,200. This substance has proved to be capable of hydrolyzing NAD+, as well as of suppressing the GTPase activity of transducin, which is indicative of the covalent modification (ADP-ribosylyzing) of GTP-binding protein under the action of B. pertussis toxin. Two methods for the isolation of B. pertussis toxin (from liquid and solid growth media), as well as the isolation of the toxin from different B. pertussis strains, are evaluated.

UI MeSH Term Description Entries
D007964 Leukocytosis A transient increase in the number of leukocytes in a body fluid. Pleocytosis,Leukocytoses,Pleocytoses
D008808 Mice, Inbred CBA An inbred strain of mouse that is widely used in BIOMEDICAL RESEARCH. Mice, CBA,Mouse, CBA,Mouse, Inbred CBA,CBA Mice,CBA Mice, Inbred,CBA Mouse,CBA Mouse, Inbred,Inbred CBA Mice,Inbred CBA Mouse
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D008970 Molecular Weight The sum of the weight of all the atoms in a molecule. Molecular Weights,Weight, Molecular,Weights, Molecular
D009243 NAD A coenzyme composed of ribosylnicotinamide 5'-diphosphate coupled to adenosine 5'-phosphate by pyrophosphate linkage. It is found widely in nature and is involved in numerous enzymatic reactions in which it serves as an electron carrier by being alternately oxidized (NAD+) and reduced (NADH). (Dorland, 27th ed) Coenzyme I,DPN,Diphosphopyridine Nucleotide,Nadide,Nicotinamide-Adenine Dinucleotide,Dihydronicotinamide Adenine Dinucleotide,NADH,Adenine Dinucleotide, Dihydronicotinamide,Dinucleotide, Dihydronicotinamide Adenine,Dinucleotide, Nicotinamide-Adenine,Nicotinamide Adenine Dinucleotide,Nucleotide, Diphosphopyridine
D010566 Virulence Factors, Bordetella A set of BACTERIAL ADHESINS and TOXINS, BIOLOGICAL produced by BORDETELLA organisms that determine the pathogenesis of BORDETELLA INFECTIONS, such as WHOOPING COUGH. They include filamentous hemagglutinin; FIMBRIAE PROTEINS; pertactin; PERTUSSIS TOXIN; ADENYLATE CYCLASE TOXIN; dermonecrotic toxin; tracheal cytotoxin; Bordetella LIPOPOLYSACCHARIDES; and tracheal colonization factor. Bordetella Virulence Factors,Agglutinogen 2, Bordetella Pertussis,Bordetella Virulence Determinant,LFP-Hemagglutinin,LP-HA,Leukocytosis-Promoting Factor Hemagglutinin,Lymphocytosis-Promoting Factor-Hemagglutinin,Pertussis Agglutinins,Agglutinins, Pertussis,Determinant, Bordetella Virulence,Factor Hemagglutinin, Leukocytosis-Promoting,Factor-Hemagglutinin, Lymphocytosis-Promoting,Factors, Bordetella Virulence,Hemagglutinin, Leukocytosis-Promoting Factor,LFP Hemagglutinin,LP HA,Leukocytosis Promoting Factor Hemagglutinin,Lymphocytosis Promoting Factor Hemagglutinin,Virulence Determinant, Bordetella
D006384 Hemagglutination The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL). Hemagglutinations
D006632 Histamine An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter. Ceplene,Histamine Dihydrochloride,Histamine Hydrochloride,Peremin
D006868 Hydrolysis The process of cleaving a chemical compound by the addition of a molecule of water.
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva
Production and purification of Bordetella pertussis toxin.
May 1997, Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology,
A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva
Production and properties of Bordetella pertussis heat-labile toxin.
February 1984, Journal of medical microbiology,
A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva
Antigens of Bordetella pertussis. II. Purification of heat-labile toxin.
August 1962, Journal of bacteriology,
A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva
Purification and characterization of the heat-labile toxin of Bordetella pertussis.
October 1991, Infection and immunity,
A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva
Adenylate cyclase toxin of Bordetella pertussis: production, purification, and partial characterization.
November 1990, Infection and immunity,
A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva
Secretion of Pertussis Toxin from Bordetella pertussis.
August 2021, Toxins,
A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva
Rapid purification of Bordetella pertussis toxin by alternating affinity and hydrophobic chromatography.
December 1986, Analytical biochemistry,
A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva
Bordetella pertussis Strain Lacking Pertactin and Pertussis Toxin.
February 2016, Emerging infectious diseases,
A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva
Adenylate cyclase toxin from Bordetella pertussis. Identification and purification of the holotoxin molecule.
November 1989, The Journal of biological chemistry,
A A Gureeva, and G Iu Grigorian, and V O Rybin, and N A Pereverzev, and I A Lapaeva
Simple, efficient purification of filamentous hemagglutinin and pertussis toxin from Bordetella pertussis by hydrophobic and affinity interaction.
May 1990, Journal of clinical microbiology,
Copied contents to your clipboard!