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A radiolabeled synthetic oligonucleotide was evaluated as a diagnostic probe specific for Plasmodium falciparum using blood samples lysed directly on nitrocellulose filters. The probe technique successfully diagnosed malaria in experimentally infected chimpanzees that had 0.001% parasitemias (50 parasites/microliter) as determined by blood smears, and in 1 chimpanzee whose blood smear was negative, but whose blood was culture-positive for P. falciparum. In a double blind study of 50 patient samples from the Philippines, the probe results correlated well with blood smear results when the autoradiographs were read after 4-8 hr exposure. The results indicate that the oligonucleotide probe may be useful in the rapid and specific diagnosis of P. falciparum infection.
C M Mucenski, and
P Guerry, and
M Buesing, and
A Szarfman, and
J Trosper, and
D Walliker, and
G Watt, and
R Sangalang, and
C P Ranoa, and
M Tuazon
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C M Mucenski, and
P Guerry, and
M Buesing, and
A Szarfman, and
J Trosper, and
D Walliker, and
G Watt, and
R Sangalang, and
C P Ranoa, and
M Tuazon
January 1997,
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C M Mucenski, and
P Guerry, and
M Buesing, and
A Szarfman, and
J Trosper, and
D Walliker, and
G Watt, and
R Sangalang, and
C P Ranoa, and
M Tuazon
July 1987,
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P Guerry, and
M Buesing, and
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J Trosper, and
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G Watt, and
R Sangalang, and
C P Ranoa, and
M Tuazon
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C M Mucenski, and
P Guerry, and
M Buesing, and
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J Trosper, and
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R Sangalang, and
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M Tuazon
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March 1990,
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