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This study utilized uniform incubation conditions and demonstrated heterogeneity in the effects of various agents on 125-insulin binding and intracellular accumulation in five cell types. The cells used in this study included rat adipocytes, H4IIEC3 cultured hepatoma cells, normal human fibroblasts, and 3T3-L1 preadipocytes and adipocytes. Bacitracin increased insulin binding to rat adipocytes but inhibited binding to all other cells. Chloroquine increased total cell-associated insulin in all cells except H4IIEC3 hepatoma cells. Methylamine and dansylcadaverine increased or decreased binding depending on cell type. Similar heterogeneity was found in the intracellular accumulation of insulin. Under control conditions, intracellular insulin at steady state varied from 16 to 52% of the total cell-associated insulin. Bacitracin decreased intracellular accumulation of insulin in 3T3-L1 adipocytes, preadipocytes, and human fibroblasts but increased accumulation in rat adipocytes. Chloroquine increased insulin accumulation in all cell types except H411EC3 hepatoma cells. Both methylamine and dansylcadaverine increased intracellular insulin in rat adipocytes and decreased accumulation in human fibroblasts. These results provide additional evidence of heterogeneity in insulin and/or insulin receptor processing among different cell types.
R M Smith, and
T S Gansler, and
N H Laudenslager, and
N Shah, and
L Jarett
January 1987,
Biochemical and biophysical research communications,
R M Smith, and
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N H Laudenslager, and
N Shah, and
L Jarett
January 1993,
Obesity research,
R M Smith, and
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N H Laudenslager, and
N Shah, and
L Jarett
May 1991,
The Journal of steroid biochemistry and molecular biology,
R M Smith, and
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N H Laudenslager, and
N Shah, and
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November 2021,
Biochemical pharmacology,
R M Smith, and
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N H Laudenslager, and
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L Jarett
March 1997,
Molecular and cellular biology,
R M Smith, and
T S Gansler, and
N H Laudenslager, and
N Shah, and
L Jarett
October 1985,
Journal of endocrinological investigation,
R M Smith, and
T S Gansler, and
N H Laudenslager, and
N Shah, and
L Jarett
April 2001,
The Journal of biological chemistry,
R M Smith, and
T S Gansler, and
N H Laudenslager, and
N Shah, and
L Jarett
June 1969,
Journal of the National Cancer Institute,
R M Smith, and
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L Jarett
March 1990,
Journal of cellular physiology,
R M Smith, and
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Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic],
