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Specialized transducing phages lambda asn harboring chromosomal DNA and genetic markers on either side of the asn gene were isolated. Phages carrying chromosomal DNA counterclockwise of the asn gene can upon infection establish themselves as self-replicating plasmids in asn, recA hosts lysogenic for lambda. It is concluded that this bypassing of normal lambda immunity is due to the presence of the chromosomal replication origin, oriC, in this class of phages. Genetic analysis and the determination of restriction endonuclease cleavage patterns of the different lambda asn lead to the allocation of oriC within 1.5 megadaltons of the asn gene towards the uncA, uncB genes at 82 min on the genetic map of E. coli. The clockwise order of genes on the chromosomes is found to be: bglB, (pst, glmS), (uncA, uncB), oriC, asn, trkD, rbs, rrnC, ilv.
K von Meyenburg, and
F G Hansen, and
L D Nielsin, and
E Riise
January 1981,
Genetika,
K von Meyenburg, and
F G Hansen, and
L D Nielsin, and
E Riise
January 1980,
Molecular & general genetics : MGG,
K von Meyenburg, and
F G Hansen, and
L D Nielsin, and
E Riise
January 1980,
Molecular & general genetics : MGG,
K von Meyenburg, and
F G Hansen, and
L D Nielsin, and
E Riise
January 1975,
Proceedings of the National Academy of Sciences of the United States of America,
K von Meyenburg, and
F G Hansen, and
L D Nielsin, and
E Riise
January 1984,
Advances in experimental medicine and biology,
K von Meyenburg, and
F G Hansen, and
L D Nielsin, and
E Riise
March 1987,
Nucleic acids research,
K von Meyenburg, and
F G Hansen, and
L D Nielsin, and
E Riise
July 1978,
Molecular & general genetics : MGG,
K von Meyenburg, and
F G Hansen, and
L D Nielsin, and
E Riise
June 1971,
Journal of molecular biology,
K von Meyenburg, and
F G Hansen, and
L D Nielsin, and
E Riise
December 1976,
FEBS letters,
K von Meyenburg, and
F G Hansen, and
L D Nielsin, and
E Riise
September 1981,
Gene,
