Surface urban heat islands (SUHIs) are one of the most studied phenomena in urban climates because they generate problems for the well-being of the urban population. This study analyzed the thermal comfort conditions at microclimate scale and SUHI for João Pessoa city, Brazil. Micrometeorological data (temperature and air humidity data) collected at 10 stations in 2011 and 2018 were used to calculate Thom's discomfort index (TDI) for João Pessoa city. Satellite images from Landsat 5/TM for 1991, 2006, and 2010 and Landsat 8/OLI for 2018 were used for land use and land cover classification and to identify SUHI. The obtained results highlighted that the SUHI area in João Pessoa city was 26 km2 and that almost half of the heat island area was concentrated in the Geisel, Aeroclube, Valentina, Distrito Industrial, Cristo Redentor, and Mangabeira neighborhoods. Regarding the micrometeorological data, higher values were obtained for 2018 in the dry periods (summer) and during the day. Based on the results, a considerable increase in discomfort during the daytime was observed in urbanized areas of the city from 2010 - 2018 due to the increase in the average temperature in João Pessoa between 1991 and 2018.