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Early (twice) and late (eighth) passage nonestablished rat embryo fibroblasts exhibit dramatically different responses to microinjection of the oncogenic form (T24) of the ras protein. Late passage cells respond like established cultures in that they both synthesize DNA and divide in response to the oncogenic protein. However, early passage cells are shown to be much less responsive to the introduction of this protein in terms of the threshold concentration required to elicit a mitogenic response. Furthermore, conditioned medium from late passage cells can both potentiate the effect of ras in early passage cells and stimulate colony formation in soft agar. In addition, we show that human cells can respond to microinjection of the ras oncogene protein by synthesizing DNA.
N F Sullivan, and
R W Sweet, and
M Rosenberg, and
J R Feramisco
October 1988,
Oncogene,
N F Sullivan, and
R W Sweet, and
M Rosenberg, and
J R Feramisco
October 1985,
Cell,
N F Sullivan, and
R W Sweet, and
M Rosenberg, and
J R Feramisco
August 1987,
Journal of virology,
N F Sullivan, and
R W Sweet, and
M Rosenberg, and
J R Feramisco
January 1988,
Neoplasma,
N F Sullivan, and
R W Sweet, and
M Rosenberg, and
J R Feramisco
November 1990,
Virology,
N F Sullivan, and
R W Sweet, and
M Rosenberg, and
J R Feramisco
May 1988,
Oncogene,
N F Sullivan, and
R W Sweet, and
M Rosenberg, and
J R Feramisco
May 1993,
Radiation research,
N F Sullivan, and
R W Sweet, and
M Rosenberg, and
J R Feramisco
October 1992,
Oncogene,
N F Sullivan, and
R W Sweet, and
M Rosenberg, and
J R Feramisco
April 1988,
Biochemical and biophysical research communications,
N F Sullivan, and
R W Sweet, and
M Rosenberg, and
J R Feramisco
August 1993,
Biochemical and biophysical research communications,
