-transparent.png){kind=logo}
An enzyme-linked immunosorbent assay for the detection of human antibodies to Chlamydiae is described which exploits the cross-react properties between the genus-specific antigen of Chlamydiae and the ReLPS constituent of the outer membrane of a Salmonella minnesota mutant. Of 100 random sera tested by ELISA-ReLPS and immunofluorescence 78% showed an absolute correlation, 15% were positive in immunofluorescence and negative in ELISA and 7% were positive in ELISA and negative in immunofluorescence. Furthermore results obtained by the ELISA-ReLPS on 55 sera from patients with clinical evidence of Chlamydiae infection correlated well with the values obtained by an ELISA using Chlamydia-coated microtitration plates and by two immunofluorescence tests using Chlamydia trachomatis and Chlamydia psittaci as antigens. The method described here is sensitive, simple, reproducible and may be employed for epidemiological and pathogenetic studies of chlamydial infections.
F Lema, and
S Everaere, and
C Rougeot, and
V Fuentes, and
E Ezan, and
F Dray, and
A Bussard
January 2002,
Journal of pharmacological and toxicological methods,
F Lema, and
S Everaere, and
C Rougeot, and
V Fuentes, and
E Ezan, and
F Dray, and
A Bussard
January 1993,
The Canadian veterinary journal = La revue veterinaire canadienne,
F Lema, and
S Everaere, and
C Rougeot, and
V Fuentes, and
E Ezan, and
F Dray, and
A Bussard
February 1986,
Journal of immunological methods,
F Lema, and
S Everaere, and
C Rougeot, and
V Fuentes, and
E Ezan, and
F Dray, and
A Bussard
July 1985,
Acta virologica,
F Lema, and
S Everaere, and
C Rougeot, and
V Fuentes, and
E Ezan, and
F Dray, and
A Bussard
February 1999,
The Journal of infectious diseases,
F Lema, and
S Everaere, and
C Rougeot, and
V Fuentes, and
E Ezan, and
F Dray, and
A Bussard
May 1993,
Journal of virological methods,
F Lema, and
S Everaere, and
C Rougeot, and
V Fuentes, and
E Ezan, and
F Dray, and
A Bussard
January 2011,
PloS one,
F Lema, and
S Everaere, and
C Rougeot, and
V Fuentes, and
E Ezan, and
F Dray, and
A Bussard
April 1985,
Immunitat und Infektion,
F Lema, and
S Everaere, and
C Rougeot, and
V Fuentes, and
E Ezan, and
F Dray, and
A Bussard
January 2009,
PloS one,
F Lema, and
S Everaere, and
C Rougeot, and
V Fuentes, and
E Ezan, and
F Dray, and
A Bussard
August 2005,
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
