Regeneration from half lower arms in the axolotl. 1986

P Wigmore

A technique involving grafting of pieces of skin from the head onto the limb in order to isolate halves of the limb is described. This technique was used to isolate posterior, anterior, dorsal and ventral halves of the lower arm. All halves produced regenerates but no part of the limb was able to produce a high proportion of regenerates with a complete pattern of skeletal structures. Posterior half stumps regenerated limbs with a mean digit number of 2.7 and had a normal dorsoventral muscle pattern. Anterior half stumps produced a high proportion of single-digit regenerates and had a mean digit number of 1.3. Dorsal and ventral half stumps regenerated limbs with a mean digit number of 2.8 and 2.3 respectively. Hypomorphic regenerates from dorsal and ventral half stumps often had only dorsal or ventral muscle. These results are in contrast to those from the upper arm (Wigmore & Holder, 1985) where a complete skeletal and muscular pattern regenerated from posterior and dorsal halves and hypomorphic regenerates were obtained from anterior and ventral half limbs.

UI MeSH Term Description Entries
D008722 Methods A series of steps taken in order to conduct research. Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D012038 Regeneration The physiological renewal, repair, or replacement of tissue. Endogenous Regeneration,Regeneration, Endogenous,Regenerations
D005552 Forelimb A front limb of a quadruped. (The Random House College Dictionary, 1980) Forelimbs
D000557 Ambystoma A genus of the Ambystomatidae family. The best known species are the axolotl AMBYSTOMA MEXICANUM and the closely related tiger salamander Ambystoma tigrinum. They may retain gills and remain aquatic without developing all of the adult characteristics. However, under proper changes in the environment they metamorphose. Amblystoma,Ambystoma tigrinum,Tiger Salamander,Amblystomas,Ambystomas,Salamander, Tiger,Salamanders, Tiger,Tiger Salamanders
D000558 Ambystoma mexicanum A salamander found in Mexican mountain lakes and accounting for about 30 percent of the urodeles used in research. The axolotl remains in larval form throughout its life, a phenomenon known as neoteny. Axolotl,Mexican Salamander,Ambystoma mexicanums,Axolotls,Salamander, Mexican,mexicanums, Ambystoma
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014034 Toes Any one of five terminal digits of the vertebrate FOOT. Toe
D016038 Skin Transplantation The grafting of skin in humans or animals from one site to another to replace a lost portion of the body surface skin. Dermatoplasty,Grafting, Skin,Transplantation, Skin,Dermatoplasties,Graftings, Skin,Skin Grafting,Skin Graftings,Skin Transplantations,Transplantations, Skin

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