-transparent.png){kind=logo}
Droplet interface bilayer (DIB) is a lipid bilayer formed when two lipid monolayer-coated aqueous droplets are brought in contact within an oil phase. DIBs, especially post functionalization, are a facile model system to study the biophysics of the cell membrane. Continued advances in enhancing and functionalizing DIBs to be a faithful cell membrane mimetic requires a deep understanding of the physicochemical characteristics of droplet interface bilayers. In this review, we provide a comprehensive overview of the current scientific understanding of DIB characteristics starting with the key experimental frameworks for DIB generation, visualization and functionalization. Subsequently we report experimentally measured physical, electrical and transport characteristics of DIBs across physiologically relevant lipids. Advances in simulations and mathematical modelling of DIBs are also discussed, with an emphasis on revealing principles governing the key physicochemical characteristics. Finally, we conclude the review with important outstanding questions in the field.
Y Huang, and
G G Fuller, and
V Chandran Suja
December 2008,
Molecular bioSystems,
Y Huang, and
G G Fuller, and
V Chandran Suja
May 2008,
Journal of the American Chemical Society,
Y Huang, and
G G Fuller, and
V Chandran Suja
April 2014,
Soft matter,
Y Huang, and
G G Fuller, and
V Chandran Suja
March 2017,
Biomicrofluidics,
Y Huang, and
G G Fuller, and
V Chandran Suja
June 2018,
Physical review letters,
Y Huang, and
G G Fuller, and
V Chandran Suja
November 2020,
Biochimica et biophysica acta. Biomembranes,
Y Huang, and
G G Fuller, and
V Chandran Suja
January 2021,
Methods in molecular biology (Clifton, N.J.),
Y Huang, and
G G Fuller, and
V Chandran Suja
May 2019,
Experimental biology and medicine (Maywood, N.J.),
Y Huang, and
G G Fuller, and
V Chandran Suja
September 2016,
Scientific reports,
Y Huang, and
G G Fuller, and
V Chandran Suja
May 2014,
Proceedings of the National Academy of Sciences of the United States of America,
