Biomaterial Database

Evaluation of culture methods for isolation of group B streptococci. 1987

K M Persson, and A Forsgren

Attempts were made to isolate group B streptococci (GBS) from 382 urethral and 140 rectal specimens by overnight incubation in three variants of Todd Hewitt broth, followed by subculture on human blood agar plates. All three broths prepared contained 5% sheep blood, but one contained no antibiotics (THB), while the other two contained gentamicin and nalidixic acid at concentrations of 0.8 microgram/ml and 1.5 micrograms/ml (THBL), and 8 micrograms/ml and 15 micrograms/ml (THBH), respectively. THBL gave the highest isolation frequency both for urethral and rectal specimens. While THBH was superior to THB for isolating GBS in rectal specimens, for urethral samples they were equally effective. Direct plating of 154 specimens on blood agar was less effective than was enrichment in any of the three broths followed by plating on blood agar.

UI	MeSH Term	Description	Entries
D009268	Nalidixic Acid	A synthetic 1,8-naphthyridine antimicrobial agent with a limited bacteriocidal spectrum. It is an inhibitor of the A subunit of bacterial DNA GYRASE.	Nalidixin,Nalidixate Sodium,Nalidixate Sodium Anhydrous,Nevigramon,Sodium Nalidixic Acid, Anhydrous,Sodium Nalidixic Acid, Monohydrate,Acid, Nalidixic,Anhydrous, Nalidixate Sodium,Sodium Anhydrous, Nalidixate,Sodium, Nalidixate
D012007	Rectum	The distal segment of the LARGE INTESTINE, between the SIGMOID COLON and the ANAL CANAL.	Rectums
D003470	Culture Media	Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.	Media, Culture
D005260	Female		Females
D005839	Gentamicins	A complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.	Gentamicin Sulfate (USP),Gentamycin,G-Myticin,Garamycin,Gentacycol,Gentamicin,Gentamicin Sulfate,Gentamycins,Gentavet,Genticin,G Myticin,GMyticin,Sulfate, Gentamicin
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013292	Streptococcus agalactiae	A bacterium which causes mastitis in cattle and occasionally in man.	Streptococcus Group B
D014521	Urethra	A tube that transports URINE from the URINARY BLADDER to the outside of the body in both the sexes. It also has a reproductive function in the male by providing a passage for SPERM.	External Urethral Sphincter,External Urinary Sphincter,Internal Urethral Sphincter,Internal Urinary Sphincter,Internal Vesical Sphincter,Urethral Sphincters,External Urethral Sphincters,External Urinary Sphincters,Internal Urethral Sphincters,Internal Urinary Sphincters,Internal Vesical Sphincters,Sphincter, External Urethral,Sphincter, External Urinary,Sphincter, Internal Urethral,Sphincter, Internal Urinary,Sphincter, Internal Vesical,Sphincter, Urethral,Urethral Sphincter,Urethral Sphincter, External,Urethras,Urinary Sphincter, External,Urinary Sphincter, Internal,Vesical Sphincter, Internal