The recent works have shown the unicellular green alga Chlamydomonas reinhardtii is a relevant model for investigations of algal bioprocesses. In the current work, several media were evaluated in batch mode for a better understanding of C. reinhardtii metabolism. Nutrient-suppression using heterotrophic and mixotrophic conditions were performed. The findings showed C. reinhardtii metabolized lactose (from milk whey permeate) resulting in high biomass density (2.08 g/L) and total chlorophyll content (86.74 mg/m3). It was observed a specific growth rate of 0.023 h and 29 h for the doubling time. In sulfur-suppression, the algal growth (1.17 g/L) was reduced even though a carbon source (glucose) has been supplemented. Also, the specific growth rate (0.022 h) and the doubling time (31 h) was verified. The production of ethanol was slight and the acetic acid-suppression affected the C. reinhardtii performance providing slow cell growth (0.004 h) and high doubling time (154 h).