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An immunocytochemical and ultrastructural study of the endocrine pancreas of Sparus auratus L. (Teleostei). 1986

M E Abad, and B Agulleiro, and J H Rombout

The pancreatic endocrine cells of Sparus auratus (gilthead sea bream) are concentrated in two or three principal islets, or Brockmann bodies, and numerous smaller islets embedded in the exocrine tissue. Insulin-, glucagon-, somatostatin-, and pancreatic polypeptide (PP)-immunoreactive cells were identified in all pancreatic islets of S. auratus using an indirect immunocytochemical (PAP) method. Insulin-immunoreactive cells were found in the central region of the islets. Glucagon-immunoreactive cells could be seen at the periphery of the islets and isolated in the exocrine tissue surrounding the large principal islet. Somatostatin-immunoreactive cells were distributed throughout the islets. PP-immunoreactive cells were clustered, in a limited shallow section, being found in no other part of the large principal islet whereas, in the smaller islets, these cells were more numerous and found in the whole peripheral area. Four cell types were identified in the pancreatic islets of S. auratus by electron microscopy. A,B,D and PP cells were characterized by the shape, size, and electron density of their respective secretory granules.

UI MeSH Term Description Entries
D007120 Immunochemistry Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
D007515 Islets of Langerhans Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN. Islands of Langerhans,Islet Cells,Nesidioblasts,Pancreas, Endocrine,Pancreatic Islets,Cell, Islet,Cells, Islet,Endocrine Pancreas,Islet Cell,Islet, Pancreatic,Islets, Pancreatic,Langerhans Islands,Langerhans Islets,Nesidioblast,Pancreatic Islet
D008854 Microscopy, Electron Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen. Electron Microscopy
D005399 Fishes A group of cold-blooded, aquatic vertebrates having gills, fins, a cartilaginous or bony endoskeleton, and elongated bodies covered with scales.
D006651 Histocytochemistry Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods. Cytochemistry
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

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