Boronic acid provides faster fluorescence response to Fe3+ compared to other reported sensors, which is critical for continuous dynamic detection. Herein, we reported a novel boronic acid-based sensor 4 that could recognize Fe3+ ion in real time. After 10 equiv. of Fe3+ ion (1 mM) was added, the fluorescence of sensor 4 was immediately quenched by 96%. While other ions, including Ba2+, Ca2+, Cr2+, Cd2+, Co2+, Cs2+, Cu2+, Fe2+, K+, Li+, Mg2+, Mn2+, Na+, Ni2+ or Zn2+, respectively, did not change the fluorescence significantly. Further tests indicated that the high selectively sensing Fe3+ ion benefits from the two boronic acid functionalities in the structure. Moreover, interference experiments showed this sensor has an excellent anti-interference ability. In addition, we performed binding activity test in rabbit plasma and other real samples for practical applications, obtaining similar results. And the thin layer loading sensor 4 was also successfully applied to recognize Fe3+ ion among various ions. Therefore, 4 may serve as a potential sensor for continuous monitoring and detecting Fe3+ ion in real time.