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This study represents the first example of immunological localization of lysosomal acid phosphatase. The intracellular localization of lysosomal acid phosphatase was investigated with immunocytochemical methods at the light and electron microscopical level in cultured fibroblasts obtained from normal subjects and from a patient with I-cell disease. Double-labeling studies using fluorescence microscopy showed that acid phosphatase is present in the same organelles as other hydrolases. At the electron microscopic level in control fibroblasts acid phosphatase was found in the rough endoplasmic reticulum, lysosomes, at the plasma membrane, in vesicles just below the plasma membrane and in multivesicular bodies. This localization was comparable with that of other lysosomal enzymes tested (acid alpha-glucosidase, N-acetyl-beta-hexosaminidase, beta-galactosidase). Acid phosphatase labeling was mainly found in association with the lysosomal membrane and with membranous material present within the lysosome. In I-cell fibroblasts the label was present in the same subcellular organelles but always associated with membranous structures. We suggest that the association of acid phosphatase with membranes might explain the normal enzyme activity found in I-cell fibroblasts.
G Parenti, and
R Willemsen, and
A T Hoogeveen, and
M Verleun-Mooyman, and
J M Van Dongen, and
H Galjaard
July 1985,
The Journal of biological chemistry,
G Parenti, and
R Willemsen, and
A T Hoogeveen, and
M Verleun-Mooyman, and
J M Van Dongen, and
H Galjaard
November 1985,
European journal of cell biology,
G Parenti, and
R Willemsen, and
A T Hoogeveen, and
M Verleun-Mooyman, and
J M Van Dongen, and
H Galjaard
January 1951,
The Anatomical record,
G Parenti, and
R Willemsen, and
A T Hoogeveen, and
M Verleun-Mooyman, and
J M Van Dongen, and
H Galjaard
April 1989,
Cell structure and function,
G Parenti, and
R Willemsen, and
A T Hoogeveen, and
M Verleun-Mooyman, and
J M Van Dongen, and
H Galjaard
September 1992,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
G Parenti, and
R Willemsen, and
A T Hoogeveen, and
M Verleun-Mooyman, and
J M Van Dongen, and
H Galjaard
January 1982,
Ophthalmic research,
G Parenti, and
R Willemsen, and
A T Hoogeveen, and
M Verleun-Mooyman, and
J M Van Dongen, and
H Galjaard
November 1985,
Collagen and related research,
G Parenti, and
R Willemsen, and
A T Hoogeveen, and
M Verleun-Mooyman, and
J M Van Dongen, and
H Galjaard
February 1987,
Cell biology international reports,
G Parenti, and
R Willemsen, and
A T Hoogeveen, and
M Verleun-Mooyman, and
J M Van Dongen, and
H Galjaard
February 1994,
The Journal of biological chemistry,
G Parenti, and
R Willemsen, and
A T Hoogeveen, and
M Verleun-Mooyman, and
J M Van Dongen, and
H Galjaard
February 1986,
Clinical chemistry,
