Newer minimally invasive treatment options for improvement in appearance of dental fluorosis stains are gaining popularity in recent years. To evaluate and compare the clinical success in esthetic improvement of resin infiltration, in-office bleaching with 35% hydrogen peroxide, enamel microabrasion, and resin infiltration with double infiltrant application on nonpitted fluorosis stains. Seventy two patients aged between 6 and 12 years with nonpitted dental fluorosis stains on the upper anterior teeth were randomly selected and divided into four interventional groups: group 1- resin infiltration (RI), group 2- in-office bleaching using 35% hydrogen peroxide (B), group 3- enamel microabrasion (M), and group 4- resin infiltration with double infiltrant application (2RI). In each group, standardized photographs were taken preoperatively, immediate postoperatively and after 1, 3, and 6 months time interval postoperatively to assess the esthetic improvement (EI) and changes in white/brown surface opacities/stains (SC) using a Visual Assessment Scale (VAS). RI and 2RI showed statistically significant results (p ≤0.05) followed by microabrasion and bleaching, for both the evaluation criteria (EI and SC) at all the follow-up intervals. Resin infiltration is a promising procedure that demonstrated remarkable clinical success for improvement in esthetics of nonpitted fluorosis stains with stable long-term positive outcome. Garg I, Kumar A, Kumar A. Comparative Evaluation of Esthetic Improvement of Resin Infiltration (RI) In-office Bleaching (B) Enamel Microabrasion (M) and Resin Infiltration with Double Application of Infiltrant (2RI) on Non-pitted Fluorosis Stains: A Randomized Six-month Interventional Study. Int J Clin Pediatr Dent 2022;15(1):20-34.