3D chromatin remodelling in the germ line modulates genome evolutionary plasticity. 2022

Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.

Chromosome folding has profound impacts on gene regulation, whose evolutionary consequences are far from being understood. Here we explore the relationship between 3D chromatin remodelling in mouse germ cells and evolutionary changes in genome structure. Using a comprehensive integrative computational analysis, we (i) reconstruct seven ancestral rodent genomes analysing whole-genome sequences of 14 species representatives of the major phylogroups, (ii) detect lineage-specific chromosome rearrangements and (iii) identify the dynamics of the structural and epigenetic properties of evolutionary breakpoint regions (EBRs) throughout mouse spermatogenesis. Our results show that EBRs are devoid of programmed meiotic DNA double-strand breaks (DSBs) and meiotic cohesins in primary spermatocytes, but are associated in post-meiotic cells with sites of DNA damage and functional long-range interaction regions that recapitulate ancestral chromosomal configurations. Overall, we propose a model that integrates evolutionary genome reshuffling with DNA damage response mechanisms and the dynamic spatial genome organisation of germ cells.

UI MeSH Term Description Entries
D008297 Male Males
D008540 Meiosis A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells. M Phase, Meiotic,Meiotic M Phase,M Phases, Meiotic,Meioses,Meiotic M Phases,Phase, Meiotic M,Phases, Meiotic M
D002843 Chromatin The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell. Chromatins
D005854 Germ Cells The reproductive cells in multicellular organisms at various stages during GAMETOGENESIS. Gamete,Gametes,Germ-Line Cells,Germ Line,Cell, Germ,Cell, Germ-Line,Cells, Germ,Cells, Germ-Line,Germ Cell,Germ Line Cells,Germ Lines,Germ-Line Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013091 Spermatogenesis The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA. Spermatocytogenesis,Spermiogenesis
D016678 Genome The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA. Genomes
D042002 Chromatin Assembly and Disassembly The mechanisms effecting establishment, maintenance, and modification of that specific physical conformation of CHROMATIN determining the transcriptional accessibility or inaccessibility of the DNA. Chromatin Remodeling,Chromatin Assembly,Chromatin Disassembly,Chromatin Modeling,Chromatin Disassemblies,Disassembly, Chromatin,Remodeling, Chromatin
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D053903 DNA Breaks, Double-Stranded Interruptions in the sugar-phosphate backbone of DNA, across both strands adjacently. Double-Stranded DNA Breaks,Double-Strand DNA Breaks,Double-Stranded DNA Break,Break, Double-Strand DNA,Break, Double-Stranded DNA,Breaks, Double-Strand DNA,Breaks, Double-Stranded DNA,DNA Break, Double-Strand,DNA Break, Double-Stranded,DNA Breaks, Double Stranded,DNA Breaks, Double-Strand,Double Strand DNA Breaks,Double Stranded DNA Break,Double Stranded DNA Breaks,Double-Strand DNA Break

Related Publications

Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
March 2005, Nature,
Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
December 2021, Nature,
Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
October 2014, Development (Cambridge, England),
Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
January 2017, Cold Spring Harbor symposia on quantitative biology,
Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
June 2004, Research in microbiology,
Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
May 2021, Nature communications,
Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
May 2022, Trends in genetics : TIG,
Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
July 2012, Cardiovascular research,
Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
February 2011, Nature communications,
Lucía Álvarez-González, and Frances Burden, and Dadakhalandar Doddamani, and Roberto Malinverni, and Emma Leach, and Cristina Marín-García, and Laia Marín-Gual, and Albert Gubern, and Covadonga Vara, and Andreu Paytuví-Gallart, and Marcus Buschbeck, and Peter J I Ellis, and Marta Farré, and Aurora Ruiz-Herrera
August 1992, Human gene therapy,
Copied contents to your clipboard!