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The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation of A. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population. In transformation of the strain GV3100 with the P type plasmid RP4 a maximum frequency of 3.5 10(-7) transformants per total recipient population was obtained. Agrobacterium Ti-plasmids were introduced in the strain GV3100 with a maximal efficiency of 4.5 10(-8). These experiments provide further evidence that the Ti-plasmid is responsible for the oncogenic properties of A tumefaciens and for its capacity to induce "opine" synthesis in Crown-gall plant cells.
M Holsters, and
D de Waele, and
A Depicker, and
E Messens, and
M van Montagu, and
J Schell
July 1972,
Journal of virology,
M Holsters, and
D de Waele, and
A Depicker, and
E Messens, and
M van Montagu, and
J Schell
January 1998,
Methods in molecular biology (Clifton, N.J.),
M Holsters, and
D de Waele, and
A Depicker, and
E Messens, and
M van Montagu, and
J Schell
December 1999,
Shi yan sheng wu xue bao,
M Holsters, and
D de Waele, and
A Depicker, and
E Messens, and
M van Montagu, and
J Schell
June 1985,
The EMBO journal,
M Holsters, and
D de Waele, and
A Depicker, and
E Messens, and
M van Montagu, and
J Schell
January 2019,
Methods in molecular biology (Clifton, N.J.),
M Holsters, and
D de Waele, and
A Depicker, and
E Messens, and
M van Montagu, and
J Schell
February 1996,
Proceedings of the National Academy of Sciences of the United States of America,
M Holsters, and
D de Waele, and
A Depicker, and
E Messens, and
M van Montagu, and
J Schell
June 2015,
Genetics and molecular research : GMR,
M Holsters, and
D de Waele, and
A Depicker, and
E Messens, and
M van Montagu, and
J Schell
November 2006,
Plant cell reports,
M Holsters, and
D de Waele, and
A Depicker, and
E Messens, and
M van Montagu, and
J Schell
May 1990,
Gene,
M Holsters, and
D de Waele, and
A Depicker, and
E Messens, and
M van Montagu, and
J Schell
August 2004,
Medical mycology,
