Biomaterial Database

Monoclonal autoantibodies from rats with inherited retinal dystrophy. 1986

D M Reid, and J V Forrester, and A M Campbell

The Royal College of Surgeons (RCS) rat carries a recessive gene for retinal dystrophy and is used as an experimental model for human retinal degenerative conditions such as retinitis pigmentosa (RP). It has been suggested that an autoimmune response to retinal antigens may contribute to the retinal degenerative processes. In this study, fusions of RCS rat splenic lymphocytes with the rat Y3 Ag 1.2.3 myeloma cell line, resulted in primary fusion cultures, some of which were positive to retinal S-antigen and others to insoluble components of rod outer segments. Three fully cloned IgM monoclonal antibodies were generated and the reaction of 2 of them to S-antigen and opsin was confirmed by immunoblotting.

UI	MeSH Term	Description	Entries
D012162	Retinal Degeneration	A retrogressive pathological change in the retina, focal or generalized, caused by genetic defects, inflammation, trauma, vascular disease, or aging. Degeneration affecting predominantly the macula lutea of the retina is MACULAR DEGENERATION. (Newell, Ophthalmology: Principles and Concepts, 7th ed, p304)	Degeneration, Retinal,Degenerations, Retinal,Retinal Degenerations
D005136	Eye Proteins	PROTEINS derived from TISSUES of the EYE.	Proteins, Eye
D006825	Hybridomas	Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.	Hybridoma
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000911	Antibodies, Monoclonal	Antibodies produced by a single clone of cells.	Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D000941	Antigens	Substances that are recognized by the immune system and induce an immune reaction.	Antigen
D001323	Autoantibodies	Antibodies that react with self-antigens (AUTOANTIGENS) of the organism that produced them.	Autoantibody
D012374	Rod Cell Outer Segment	The portion of a retinal rod cell situated between the ROD INNER SEGMENT and the RETINAL PIGMENT EPITHELIUM. It contains a stack of photosensitive disk membranes laden with RHODOPSIN.	Rod Outer Segment,Rod Outer Segments,Outer Segment, Rod,Outer Segments, Rod
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D019393	Arrestin	A 48-Kd protein of the outer segment of the retinal rods and a component of the phototransduction cascade. Arrestin quenches G-protein activation by binding to phosphorylated photolyzed rhodopsin. Arrestin causes experimental autoimmune uveitis when injected into laboratory animals.	Retinal S-Antigen,S-Antigen, Retinal,Retinal S Antigen,S Antigen, Retinal