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Pyruvate kinase (PK) has four isozymes (L,R,M1,M2) that are encoded mainly by two different genes. We isolated a cDNA clone from a Japanese adult liver lambda gt10 cDNA library by using a rat liver(L)-type PK cDNA probe. One positively hybridizing clone, hlPK-1, which contained a 1,049-base pair cDNA insert, was subjected to DNA sequence analysis. Comparisons of the sequence data with the rat PK cDNAs indicated that the cDNA encoded information for the carboxyl terminal 105 amino acids of a human L-type PK and a 3' untranslated region of 734 nucleotides. Furthermore, the karyotype analysis of several human-mouse hybrid cells and Southern blot analysis of DNAs of the hybrids with a hlPK-1 indicated that the human L-type PK gene is located on chromosome 1.
K Tani, and
H Fujii, and
H Tsutsumi, and
J Sukegawa, and
K Toyoshima, and
M C Yoshida, and
T Noguchi, and
T Tanaka, and
S Miwa
October 1999,
European journal of biochemistry,
K Tani, and
H Fujii, and
H Tsutsumi, and
J Sukegawa, and
K Toyoshima, and
M C Yoshida, and
T Noguchi, and
T Tanaka, and
S Miwa
July 1998,
Genomics,
K Tani, and
H Fujii, and
H Tsutsumi, and
J Sukegawa, and
K Toyoshima, and
M C Yoshida, and
T Noguchi, and
T Tanaka, and
S Miwa
December 1983,
The Journal of biological chemistry,
K Tani, and
H Fujii, and
H Tsutsumi, and
J Sukegawa, and
K Toyoshima, and
M C Yoshida, and
T Noguchi, and
T Tanaka, and
S Miwa
October 1989,
The Journal of biological chemistry,
K Tani, and
H Fujii, and
H Tsutsumi, and
J Sukegawa, and
K Toyoshima, and
M C Yoshida, and
T Noguchi, and
T Tanaka, and
S Miwa
March 1996,
Biochemical and biophysical research communications,
K Tani, and
H Fujii, and
H Tsutsumi, and
J Sukegawa, and
K Toyoshima, and
M C Yoshida, and
T Noguchi, and
T Tanaka, and
S Miwa
May 1991,
FEBS letters,
K Tani, and
H Fujii, and
H Tsutsumi, and
J Sukegawa, and
K Toyoshima, and
M C Yoshida, and
T Noguchi, and
T Tanaka, and
S Miwa
November 1990,
European journal of biochemistry,
K Tani, and
H Fujii, and
H Tsutsumi, and
J Sukegawa, and
K Toyoshima, and
M C Yoshida, and
T Noguchi, and
T Tanaka, and
S Miwa
June 1991,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
K Tani, and
H Fujii, and
H Tsutsumi, and
J Sukegawa, and
K Toyoshima, and
M C Yoshida, and
T Noguchi, and
T Tanaka, and
S Miwa
July 1994,
Biochemical and biophysical research communications,
K Tani, and
H Fujii, and
H Tsutsumi, and
J Sukegawa, and
K Toyoshima, and
M C Yoshida, and
T Noguchi, and
T Tanaka, and
S Miwa
April 1993,
Genomics,
