[Sample technique in cytology (author's transl)]. 1978

O Leder

Modern theory and experience question the reliability and suitability of sampling per judgment being the method for the evaluation of the percentage of cells in clinical cytology till now. For the reduction of this gap empirical studies were undertaken in order to receive proper estimates of blood smears. A simple method for the differential blood count is to take 30 or more strips of the width of the field of vision of the microscope perpendicular to the smearing direction as sampling units at random or by systemtaic selection with a random starting point. For the class of cells under consideration as well as for whole the cell population on the slide, the coefficient of variation of the mean of the cells within different strips has to be less than 0,1. Smears of a width of 10 mm instead of the usual 22 mm seems to be preferable. Under such conditions more than 1.000 cells are to be counted for a reliable estimate of the more frequent classes of cells. It is not supposed that this tedious method will be carried out by technicians but modern apparatus are presumed to give consistent estimates in such a way.

UI MeSH Term Description Entries
D007962 Leukocytes White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES). Blood Cells, White,Blood Corpuscles, White,White Blood Cells,White Blood Corpuscles,Blood Cell, White,Blood Corpuscle, White,Corpuscle, White Blood,Corpuscles, White Blood,Leukocyte,White Blood Cell,White Blood Corpuscle
D001773 Blood Cells The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM. Blood Corpuscles,Blood Cell,Blood Corpuscle,Cell, Blood,Cells, Blood,Corpuscle, Blood,Corpuscles, Blood
D001800 Blood Specimen Collection The taking of a blood sample to determine its character as a whole, to identify levels of its component cells, chemicals, gases, or other constituents, to perform pathological examination, etc. Blood Specimen Collections,Collection, Blood Specimen,Collections, Blood Specimen,Specimen Collection, Blood,Specimen Collections, Blood
D003581 Cytodiagnosis Diagnosis of the type and, when feasible, the cause of a pathologic process by means of microscopic study of cells in an exudate or other form of body fluid. (Stedman, 26th ed) Cytodiagnoses
D006652 Histological Techniques Methods of preparing tissue for examination and study of the origin, structure, function, or pathology. Histologic Technic,Histologic Technics,Histologic Technique,Histologic Techniques,Histological Technics,Technic, Histologic,Technics, Histologic,Technique, Histologic,Techniques, Histologic,Histological Technic,Histological Technique,Technic, Histological,Technics, Histological,Technique, Histological,Techniques, Histological
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

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