-transparent.png){kind=logo}
An aldehyde derivative of riboflavin was covalently attached by reductive alkylation to soluble polycationic supports. The flavopolymers so obtained were stable under operational conditions. The catalytic efficiency towards oxidation of NADH by these flavopolymers was demonstrated, and the kinetic parameters (Km and kcat) revealed an overall catalytic efficiency (kcat/Km) 185-fold greater compared to riboflavin. Various factors affecting the chemical regeneration of NAD+ from NADH such as pH, ionic strength, nature of the buffer etc. were studied. The most interesting result was the highly favourable influence of borate ions which increased the reaction rate by a factor 2-4 compared to the other buffers. The flavopolymers are very effective for in situ recycling of NAD(P)+. With up to 300-fold NADH----NAD+ conversions for the system using yeast alcohol dehydrogenase and up to 1500-fold NADPH----NADP+ regenerations for the system using glucose-6-phosphate dehydrogenase. These flavopolymers are superior to previous chemical recycling systems.
F Montaine, and
J P Lenders, and
R R Crichton
October 2005,
Applied and environmental microbiology,
F Montaine, and
J P Lenders, and
R R Crichton
August 2011,
Organic & biomolecular chemistry,
F Montaine, and
J P Lenders, and
R R Crichton
September 2018,
World journal of microbiology & biotechnology,
F Montaine, and
J P Lenders, and
R R Crichton
May 2006,
Combinatorial chemistry & high throughput screening,
F Montaine, and
J P Lenders, and
R R Crichton
January 1988,
Methods in enzymology,
F Montaine, and
J P Lenders, and
R R Crichton
March 1991,
Bio/technology (Nature Publishing Company),
F Montaine, and
J P Lenders, and
R R Crichton
July 2023,
ACS synthetic biology,
F Montaine, and
J P Lenders, and
R R Crichton
August 2012,
Molecules (Basel, Switzerland),
F Montaine, and
J P Lenders, and
R R Crichton
March 1986,
European journal of biochemistry,
F Montaine, and
J P Lenders, and
R R Crichton
July 1979,
The Biochemical journal,
