Biomaterial Database

Pharmacological effects of haem biosynthetic intermediates in isolated intestinal preparations. 1987

M G Cutler, and S Arrol

Spontaneous contractile activity of isolated rabbit jejunal preparations bathed in oxygenated Ringer-Locke buffered to pH 7.0 was inhibited by haemin and by all haem biosynthetic intermediates tested, there being decreases in tone or amplitude of contractions. Effects were concentration-dependent. The minimal effective concentrations were 0.4 mM for porphobilinogen, 0.6 mM for protoporphyrin IX, 0.8 mM for haemin, 3.0 mM for delta-aminolaevulinic acid (ALA), 4.5 mM for coproporphyrin I, 3.0 mM for glycine and 6.0 mM for succinate. Inhibition was short-lasting, other than for protoporphyrin (2.3 mM) and haemin (3.0 mM). Leucine at concentrations up to 12 mM had no significant effect. Inhibitory effects of ALA were followed by contractions of increased amplitude; pretreatment of preparations with indomethacin (56 microM) prevented this enhancement of contractile activity. Tone in isolated preparations of human taenia coli bathed in oxygenated Ringer-Locke was decreased by ALA (1.5-6.0 mM). A significant increase of tone followed the initial inhibitory effect. The relevance of these findings to acute porphyria is discussed.

UI	MeSH Term	Description	Entries
D007413	Intestinal Mucosa	Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.	Intestinal Epithelium,Intestinal Glands,Epithelium, Intestinal,Gland, Intestinal,Glands, Intestinal,Intestinal Gland,Mucosa, Intestinal
D007422	Intestines	The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.	Intestine
D007583	Jejunum	The middle portion of the SMALL INTESTINE, between DUODENUM and ILEUM. It represents about 2/5 of the remaining portion of the small intestine below duodenum.	Jejunums
D008297	Male		Males
D009119	Muscle Contraction	A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.	Inotropism,Muscular Contraction,Contraction, Muscle,Contraction, Muscular,Contractions, Muscle,Contractions, Muscular,Inotropisms,Muscle Contractions,Muscular Contractions
D011162	Porphobilinogen
D011524	Protoporphyrins	Porphyrins with four methyl, two vinyl, and two propionic acid side chains attached to the pyrrole rings. Protoporphyrin IX occurs in hemoglobin, myoglobin, and most of the cytochromes.
D011817	Rabbits	A burrowing plant-eating mammal with hind limbs that are longer than its fore limbs. It belongs to the family Leporidae of the order Lagomorpha, and in contrast to hares, possesses 22 instead of 24 pairs of chromosomes.	Belgian Hare,New Zealand Rabbit,New Zealand Rabbits,New Zealand White Rabbit,Rabbit,Rabbit, Domestic,Chinchilla Rabbits,NZW Rabbits,New Zealand White Rabbits,Oryctolagus cuniculus,Chinchilla Rabbit,Domestic Rabbit,Domestic Rabbits,Hare, Belgian,NZW Rabbit,Rabbit, Chinchilla,Rabbit, NZW,Rabbit, New Zealand,Rabbits, Chinchilla,Rabbits, Domestic,Rabbits, NZW,Rabbits, New Zealand,Zealand Rabbit, New,Zealand Rabbits, New,cuniculus, Oryctolagus
D003106	Colon	The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON.	Appendix Epiploica,Taenia Coli,Omental Appendices,Omental Appendix,Appendices, Omental,Appendix, Omental
D003306	Coproporphyrins	Porphyrins with four methyl and four propionic acid side chains attached to the pyrrole rings. Elevated levels of Coproporphyrin III in the urine and feces are major findings in patients with HEREDITARY COPROPORPHYRIA.