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Different development stages of porcine embryos have different tolerance to low temperature. Therefore, we took the porcine embryos after parthenogenetic activation (PA) as the model, to explore the optimal development stage for vitrification during morula (D4), early blastocyst (D5), and expanded blastocyst (D6) after PA (D0). Embryos were observed with microscope and analyzed by different staining after cryo-recovery for 24 hours. The quality of embryos was damaged after vitrification, including embryonic nuclei, DNA, cytoskeleton, and organelles. The re-expansion rate at 24 hours of D5 embryos was significantly higher than those of D4 and D6 embryos (D5 vs. D4 vs. D6, 27.620 ± 0.041 vs. 7.809 ± 0.027 vs. 13.970 ± 0.032, p < 0.05). Therefore, D5 embryos were selected as research objects to explore the effect of vitrification on lipid in vitrified embryos. The results showed that the expression levels of perilipin PLIN3 messenger RNA (mRNA) and triacylglycerol synthesis-related genes AGPAT1 and DGAT mRNA are significantly reduced (p < 0.05). Vitrification affected lipid synthesis, which might have an irreversible impact on embryonic development. In conclusion, our data demonstrated that the optimal stage of vitrification was D5 for early blastocysts.
Hongxia Xu, and
Xuguang Wang, and
Ruixin Tao, and
Jiaying Bi, and
Xu He, and
Fuquan Zhu, and
Ke Liu, and
Yinxue Xu, and
Juan Li
February 2012,
Cryobiology,
Hongxia Xu, and
Xuguang Wang, and
Ruixin Tao, and
Jiaying Bi, and
Xu He, and
Fuquan Zhu, and
Ke Liu, and
Yinxue Xu, and
Juan Li
February 2016,
Theriogenology,
Hongxia Xu, and
Xuguang Wang, and
Ruixin Tao, and
Jiaying Bi, and
Xu He, and
Fuquan Zhu, and
Ke Liu, and
Yinxue Xu, and
Juan Li
December 2020,
Cryobiology,
Hongxia Xu, and
Xuguang Wang, and
Ruixin Tao, and
Jiaying Bi, and
Xu He, and
Fuquan Zhu, and
Ke Liu, and
Yinxue Xu, and
Juan Li
November 2009,
Reproductive biomedicine online,
Hongxia Xu, and
Xuguang Wang, and
Ruixin Tao, and
Jiaying Bi, and
Xu He, and
Fuquan Zhu, and
Ke Liu, and
Yinxue Xu, and
Juan Li
September 2018,
Clinical and experimental reproductive medicine,
Hongxia Xu, and
Xuguang Wang, and
Ruixin Tao, and
Jiaying Bi, and
Xu He, and
Fuquan Zhu, and
Ke Liu, and
Yinxue Xu, and
Juan Li
September 2010,
Taiwanese journal of obstetrics & gynecology,
Hongxia Xu, and
Xuguang Wang, and
Ruixin Tao, and
Jiaying Bi, and
Xu He, and
Fuquan Zhu, and
Ke Liu, and
Yinxue Xu, and
Juan Li
January 2007,
Theriogenology,
Hongxia Xu, and
Xuguang Wang, and
Ruixin Tao, and
Jiaying Bi, and
Xu He, and
Fuquan Zhu, and
Ke Liu, and
Yinxue Xu, and
Juan Li
January 2022,
Frontiers in veterinary science,
Hongxia Xu, and
Xuguang Wang, and
Ruixin Tao, and
Jiaying Bi, and
Xu He, and
Fuquan Zhu, and
Ke Liu, and
Yinxue Xu, and
Juan Li
October 1997,
The Veterinary record,
Hongxia Xu, and
Xuguang Wang, and
Ruixin Tao, and
Jiaying Bi, and
Xu He, and
Fuquan Zhu, and
Ke Liu, and
Yinxue Xu, and
Juan Li
September 2008,
Theriogenology,
