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A method is described for quantifying the positional isomers in monounsaturated fatty acid methyl ester (FAME) fractions. The procedure involves the preparation of 2,4-dinitrophenylhydrazones (DNPH) of the fragments generated during reductive ozonolysis of FAME, class isolation of the aldehyde and aldehyde ester DNPH, and separation of the aldehyde ester derivatives by high performance liquid chromatography (HPLC). The high extinction coefficient of the DNPH provides for a sensitive assay which is linear for a large range of components over a concentration range of 0.075-5 nmol/component, and the stability of the DNPH permits the independent analysis of the aldehyde and aldehyde ester fragments generated during reductive ozonolysis. The reductive ozonolysis-DNPH-HPLC method developed is as sensitive, reproducible, and accurate as reductive ozonolysis-gas-liquid chromatography and does not suffer from some of the drawbacks of the classical procedure.
C R Caughman, and
L C Boyd, and
M Keeney, and
J Sampugna
January 1982,
Lipids,
C R Caughman, and
L C Boyd, and
M Keeney, and
J Sampugna
August 2009,
Journal of chromatography. A,
C R Caughman, and
L C Boyd, and
M Keeney, and
J Sampugna
March 2005,
Se pu = Chinese journal of chromatography,
C R Caughman, and
L C Boyd, and
M Keeney, and
J Sampugna
August 2004,
Journal of separation science,
C R Caughman, and
L C Boyd, and
M Keeney, and
J Sampugna
January 1979,
Analytical biochemistry,
C R Caughman, and
L C Boyd, and
M Keeney, and
J Sampugna
June 2011,
Analytical biochemistry,
C R Caughman, and
L C Boyd, and
M Keeney, and
J Sampugna
January 1966,
Lipids,
C R Caughman, and
L C Boyd, and
M Keeney, and
J Sampugna
November 1968,
Journal of lipid research,
C R Caughman, and
L C Boyd, and
M Keeney, and
J Sampugna
May 1979,
Journal of chromatographic science,
C R Caughman, and
L C Boyd, and
M Keeney, and
J Sampugna
June 1982,
Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology,
