-transparent.png){kind=logo}
This is the first report of parallel studies of binding indices and physiological responsiveness of the "Serotonin-two" (5-HT2) receptor on the human platelet membrane. Binding indices were measured by a microassay employing [125I]ILSD as radioligand and ketanserin to define specific binding. A single receptor population was found, characterized by a KD of 1.69 +/- 0.45 nM and Bmax of 14.5 +/- 6.0 pmol/g protein in healthy subjects. Functional responsiveness of the platelet 5-HT2 receptor complex was assessed by measurement of the extent to which serotonin (10uM) augmented platelet aggregation induced by threshold concentrations of adenosine diphosphate (ADP). A statistically significant positive correlation was found between the number of platelet 5-HT2 receptor sites (Bmax) and the magnitude of the serotonin-amplified aggregation response (r = .70, n = 38, p less than 0.001). Assessment of binding indices and physiological responsiveness of the platelet 5-HT2 receptor complex should facilitate study of age, hormonal, disease, and drug effects on 5-HT2 receptor function in human subjects.
P A McBride, and
J J Mann, and
M J Polley, and
A J Wiley, and
J A Sweeney
January 1990,
Psychopharmacology,
P A McBride, and
J J Mann, and
M J Polley, and
A J Wiley, and
J A Sweeney
September 1984,
Biochemical pharmacology,
P A McBride, and
J J Mann, and
M J Polley, and
A J Wiley, and
J A Sweeney
November 1987,
Life sciences,
P A McBride, and
J J Mann, and
M J Polley, and
A J Wiley, and
J A Sweeney
June 1991,
European journal of pharmacology,
P A McBride, and
J J Mann, and
M J Polley, and
A J Wiley, and
J A Sweeney
January 1990,
Psychopharmacology,
P A McBride, and
J J Mann, and
M J Polley, and
A J Wiley, and
J A Sweeney
January 1991,
Life sciences,
P A McBride, and
J J Mann, and
M J Polley, and
A J Wiley, and
J A Sweeney
December 1989,
Biochemical pharmacology,
P A McBride, and
J J Mann, and
M J Polley, and
A J Wiley, and
J A Sweeney
February 1984,
Thrombosis research,
P A McBride, and
J J Mann, and
M J Polley, and
A J Wiley, and
J A Sweeney
August 1997,
European journal of pharmacology,
P A McBride, and
J J Mann, and
M J Polley, and
A J Wiley, and
J A Sweeney
December 1991,
Biochemical and biophysical research communications,
