Biomaterial Database

Hepatic fatty acid profiles in aflatoxin-exposed broiler chickens. 1987

J W Merkley, and R J Maxwell, and J G Phillips, and W E Huff

This study was initiated to determine whether a specific fatty acid marker from hepatic lipids could be identified to confirm aflatoxin exposure in chickens. Male broiler chicks (240) were reared to 21 days of age on diets containing 0, 625, 1.25, 5.0, and 10.0 ppm aflatoxin. Live weights and organ weights differed significantly among the dietary groups. Total liver lipids increased significantly (P less than .001) with increasing aflatoxin levels. Separation of extracted lipids into neutral and polar fractions established that this increase in hepatic lipid was accounted for almost entirely by increases in neutral lipid fractions. A gain of 117% occurred in the weight of neutral lipids as aflatoxin increased from 0 to 10 ppm. In both neutral and polar lipid fractions subsequent chromatographic analysis of fatty acid methyl esters (FAME) showed linear increases in the monoenoic fatty acids with increasing aflatoxin. No single FAME from either lipid fraction was suitable for confirming aflatoxin exposure.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008297	Male		Males
D009929	Organ Size	The measurement of an organ in volume, mass, or heaviness.	Organ Volume,Organ Weight,Size, Organ,Weight, Organ
D002645	Chickens	Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.	Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D005227	Fatty Acids	Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)	Aliphatic Acid,Esterified Fatty Acid,Fatty Acid,Fatty Acids, Esterified,Fatty Acids, Saturated,Saturated Fatty Acid,Aliphatic Acids,Acid, Aliphatic,Acid, Esterified Fatty,Acid, Saturated Fatty,Esterified Fatty Acids,Fatty Acid, Esterified,Fatty Acid, Saturated,Saturated Fatty Acids
D000348	Aflatoxins	Furano-furano-benzopyrans that are produced by ASPERGILLUS from STERIGMATOCYSTIN. They are structurally related to COUMARINS and easily oxidized to an epoxide form to become ALKYLATING AGENTS. Members of the group include AFLATOXIN B1; aflatoxin B2, aflatoxin G1, aflatoxin G2; AFLATOXIN M1; and aflatoxin M2.	Aflatoxin
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D050356	Lipid Metabolism	Physiological processes in biosynthesis (anabolism) and degradation (catabolism) of LIPIDS.	Metabolism, Lipid