BIOMAT Database Logo BIOMAT Database Logo

Eimeria falciformis secretes extracellular vesicles to modulate proinflammatory response during interaction with mouse intestinal epithelial cells. 2022

Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.

BACKGROUND Protozoan parasite secretions can be triggered by various modified media and diverse physicochemical stressors. Equally, host-parasite interactions are known to co-opt the exchange and secretion of soluble biochemical components. Analysis of Eimeria falciformis sporozoite secretions in response to interaction with mouse intestinal epithelial cells (MIECs) may reveal parasite secretory motifs, protein composition and inflammatory activities of E. falciformis extracellular vesicles (EVs). METHODS Eimeria falciformis sporozoites were allowed to interact with inactivated MIECs. Parasite secretions were separated into EV and vesicle-free (VF) fractions by discontinuous centrifugation and ultracentrifugation. Secreted EVs were purified in an iodixanol density gradient medium and the protein composition of both EV and VF fractions were analyzed by liquid chromatoraphy-tandem mass spectroscopy. The inflammatory activities of E. falciformis sporozoite EV on MIECs were then investigated. RESULTS During the interaction of E. falciformis sporozoites with inactivated MIECs, the parasite secreted VF and vesicle-bound molecules. Eimeria falciformis vesicles are typical pathogenic protozoan EVs with a mean diameter of 264 ± 2 nm, and enclosed heat shock protein (Hsp) 70 as classical EV marker. Refractile body-associated aspartyl proteinase (or eimepsin), GAP45 and aminopeptidase were the main components of E. falciformis sporozoite EVs, while VF proteins include Hsp90, actin, Vps54 and kinases, among others. Proteomic data revealed that E. falciformis EV and VF proteins are aggregates of bioactive, antigenic and immunogenic molecules which act in concert for E. falciformis sporozoite motility, pathogenesis and survival. Moreover, in MIECs, E. falciformis EVs induced upregulation of gene expression and secretion of IL-1β, IL-6, IL-17, IL-18, MCP1 as well as pyroptosis-dependent caspase 11 and NLRP6 inflammasomes with the concomitant secretion of lactate dehydrogenase. CONCLUSIONS Eimeria falciformis sporozoite interaction with MIECs triggered the secretion of immunogenic and antigenic proteins. In addition, E. falciformis sporozoite EVs constitute parasite-associated molecular pattern that induced inflammatory response and cell death. This study offers additional insight in the secretion and protein composition of E. falciformis secretomes as well as the proinflammatory functions of E. falciformis sporozoite EVs.

UI MeSH Term Description Entries
D010271 Parasites Invertebrate organisms that live on or in another organism (the host), and benefit at the expense of the other. Traditionally excluded from definition of parasites are pathogenic BACTERIA; FUNGI; VIRUSES; and PLANTS; though they may live parasitically. Parasite
D004539 Eimeria A genus of protozoan parasites of the subclass COCCIDIA. Various species are parasitic in the epithelial cells of the liver and intestines of man and other animals. Eimerias
D004847 Epithelial Cells Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells. Adenomatous Epithelial Cells,Columnar Glandular Epithelial Cells,Cuboidal Glandular Epithelial Cells,Glandular Epithelial Cells,Squamous Cells,Squamous Epithelial Cells,Transitional Epithelial Cells,Adenomatous Epithelial Cell,Cell, Adenomatous Epithelial,Cell, Epithelial,Cell, Glandular Epithelial,Cell, Squamous,Cell, Squamous Epithelial,Cell, Transitional Epithelial,Cells, Adenomatous Epithelial,Cells, Epithelial,Cells, Glandular Epithelial,Cells, Squamous,Cells, Squamous Epithelial,Cells, Transitional Epithelial,Epithelial Cell,Epithelial Cell, Adenomatous,Epithelial Cell, Glandular,Epithelial Cell, Squamous,Epithelial Cell, Transitional,Epithelial Cells, Adenomatous,Epithelial Cells, Glandular,Epithelial Cells, Squamous,Epithelial Cells, Transitional,Glandular Epithelial Cell,Squamous Cell,Squamous Epithelial Cell,Transitional Epithelial Cell
D000067128 Extracellular Vesicles Membrane limited structures derived from cell membranes and cytoplasmic material, and released into EXTRACELLULAR SPACE. They circulate through the EXTRACELLULAR FLUID and through the peripheral blood in the MICROVASCULATURE where cells, much larger, cannot, thereby affecting a variety of intercellular communication processes. Apoptotic Bodies,Exovesicles,Apoptotic Body,Bodies, Apoptotic,Body, Apoptotic,Exovesicle,Extracellular Vesicle,Vesicle, Extracellular,Vesicles, Extracellular
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D034101 Sporozoites The product of meiotic division of zygotes in parasitic protozoa comprising haploid cells. These infective cells invade the host and undergo asexual reproduction producing MEROZOITES (or other forms) and ultimately gametocytes. Sporozoite
D040901 Proteomics The systematic study of the complete complement of proteins (PROTEOME) of organisms. Peptidomics

Related Publications

Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
Eimeria falciformis extracellular vesicles differentially express host cell lncRNAs.
December 2023, The Journal of eukaryotic microbiology,
Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
Quantitative proteomic analysis of local and systemic extracellular vesicles during Eimeria falciformis infectious cycle in the host.
September 2023, Parasites & vectors,
Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
CD8+ cells protect mice against reinfection with the intestinal parasite Eimeria falciformis.
March 2010, Microbes and infection,
Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
Murine major histocompatibility complex and immune response to Eimeria falciformis.
January 1988, Infection and immunity,
Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
Intestinal Epithelial Cell-Derived Extracellular Vesicles Modulate Hepatic Injury via the Gut-Liver Axis During Acute Alcohol Injury.
January 2020, Frontiers in pharmacology,
Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
Interaction between Campylobacter and intestinal epithelial cells leads to a different proinflammatory response in human and porcine host.
November 2014, Veterinary immunology and immunopathology,
Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
EpCAM-dependent extracellular vesicles from intestinal epithelial cells maintain intestinal tract immune balance.
October 2016, Nature communications,
Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
Human intestinal epithelial cells produce proinflammatory cytokines in response to infection in a SCID mouse-human intestinal xenograft model of amebiasis.
May 1997, Infection and immunity,
Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
Role of extracellular vesicles in the interaction between epithelial and mesenchymal cells during oviductal ciliogenesis.
January 2017, Biochemical and biophysical research communications,
Joshua Seun Olajide, and Ling Xiong, and Shunli Yang, and Zigang Qu, and Xiao Xu, and Bin Yang, and Jing Wang, and Baohong Liu, and Xueting Ma, and Jianping Cai
Corrigendum: EpCAM-dependent extracellular vesicles from intestinal epithelial cells maintain intestinal tract immune balance.
June 2017, Nature communications,
Copied contents to your clipboard!