-transparent.png){kind=logo}
Bovine oocytes matured in vivo or in vitro were evaluated after sperm-oocyte incubation for frequency of sperm penetration, frequency of male pronuclei formation, and embryonic development. The frequency of sperm penetration was not different for in vitro matured oocytes (216/295, 73%) vs. in vivo matured oocytes (119/176, 70%). However, formation of male pronuclei was reduced (p less than 0.05) for oocytes matured in vitro (149/216, 69%) vs. in vivo (104/119, 88%). Early embryonic development was evaluated 48 h after the onset of sperm-egg incubations. In vitro matured and fertilized oocytes failed to develop to the 2-cell stage (3/88, 3%), whereas oocytes matured in vivo showed normal development (23/56, 40%) to the 2- and 4-cell stage. Development to the blastocyst stage was evaluated after 5 days in ovine oviducts (in vivo). Morulae and blastocysts were obtained only after in vitro fertilization from oocytes that were in vivo-matured (recovered from oviduct, 14/56, 25%; recovered from follicle, 36/80, 45%). Oocytes that were matured in vitro and fertilized in vitro failed to develop to morulae (0/33) in vivo.
M L Leibfried-Rutledge, and
E S Critser, and
W H Eyestone, and
D L Northey, and
N L First
February 2015,
Zygote (Cambridge, England),
M L Leibfried-Rutledge, and
E S Critser, and
W H Eyestone, and
D L Northey, and
N L First
October 1987,
Journal of in vitro fertilization and embryo transfer : IVF,
M L Leibfried-Rutledge, and
E S Critser, and
W H Eyestone, and
D L Northey, and
N L First
May 1993,
Theriogenology,
M L Leibfried-Rutledge, and
E S Critser, and
W H Eyestone, and
D L Northey, and
N L First
January 1986,
Acta veterinaria Scandinavica,
M L Leibfried-Rutledge, and
E S Critser, and
W H Eyestone, and
D L Northey, and
N L First
August 1984,
Theriogenology,
M L Leibfried-Rutledge, and
E S Critser, and
W H Eyestone, and
D L Northey, and
N L First
August 1989,
Journal of animal science,
M L Leibfried-Rutledge, and
E S Critser, and
W H Eyestone, and
D L Northey, and
N L First
September 1998,
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology,
M L Leibfried-Rutledge, and
E S Critser, and
W H Eyestone, and
D L Northey, and
N L First
April 2009,
Theriogenology,
M L Leibfried-Rutledge, and
E S Critser, and
W H Eyestone, and
D L Northey, and
N L First
April 1992,
Theriogenology,
M L Leibfried-Rutledge, and
E S Critser, and
W H Eyestone, and
D L Northey, and
N L First
March 1988,
The Veterinary record,
