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We produced antiserum to insulin-like growth factor I (IGF-I), and developed a specific and sensitive radioimmunoassay (RIA) for IGF-I using the biosynthetic IGF-I. This antiserum to IGF-I was specific for IGF-I; no cross-reactivities with multiplication stimulating activity, porcine insulin or human growth hormone (hGH) were detected. The sensitivity was 10-25 pg/tube with 50% displacement at 125 pg/tube. The intra- and inter-assay coefficients of variation for IGF-I were 5.4 and 9.7%, respectively. The plasma IGF-I levels as determined by RIA in normal adults (N = 46), patients with active acromegaly (N = 31), and pituitary dwarfs (N = 31) were 21.6 +/- 1.0, 157.3 +/- 17.0, and 2.5 +/- 0.3 ng/ml (Mean +/- SEM), respectively, indicating the levels were GH-dependent. The plasma IGF-I levels were significantly increased from 2.2 +/- 0.2 to 26.5 +/- 3.2 ng/ml after hGH administrations for three consecutive days in five pituitary dwarfs. The IGF-I levels were low in patients with hypothyroidism and liver cirrhosis, but were normal in patients with chronic renal failure. These data confirm previous reports and this radioimmunoassay proves useful in evaluating plasma IGF-I levels.
M Miyakawa, and
N Hizuka, and
K Takano, and
I Tanaka, and
R Horikawa, and
N Honda, and
K Shizume
June 1995,
Cellular and molecular biology (Noisy-le-Grand, France),
M Miyakawa, and
N Hizuka, and
K Takano, and
I Tanaka, and
R Horikawa, and
N Honda, and
K Shizume
October 1990,
Endocrinologia japonica,
M Miyakawa, and
N Hizuka, and
K Takano, and
I Tanaka, and
R Horikawa, and
N Honda, and
K Shizume
May 1996,
The Journal of clinical endocrinology and metabolism,
M Miyakawa, and
N Hizuka, and
K Takano, and
I Tanaka, and
R Horikawa, and
N Honda, and
K Shizume
November 1982,
FEBS letters,
M Miyakawa, and
N Hizuka, and
K Takano, and
I Tanaka, and
R Horikawa, and
N Honda, and
K Shizume
July 2010,
Nihon rinsho. Japanese journal of clinical medicine,
M Miyakawa, and
N Hizuka, and
K Takano, and
I Tanaka, and
R Horikawa, and
N Honda, and
K Shizume
August 2005,
Nihon rinsho. Japanese journal of clinical medicine,
M Miyakawa, and
N Hizuka, and
K Takano, and
I Tanaka, and
R Horikawa, and
N Honda, and
K Shizume
January 1990,
Journal of immunoassay,
M Miyakawa, and
N Hizuka, and
K Takano, and
I Tanaka, and
R Horikawa, and
N Honda, and
K Shizume
November 2012,
Progress in orthodontics,
M Miyakawa, and
N Hizuka, and
K Takano, and
I Tanaka, and
R Horikawa, and
N Honda, and
K Shizume
August 1994,
The Journal of endocrinology,
M Miyakawa, and
N Hizuka, and
K Takano, and
I Tanaka, and
R Horikawa, and
N Honda, and
K Shizume
December 2002,
Reviews in endocrine & metabolic disorders,
